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Reactive Oxygen Species Are Required for Human Mesenchymal Stem Cells to Initiate Proliferation after the Quiescence Exit.
Lyublinskaya, O G; Borisov, Ya G; Pugovkina, N A; Smirnova, I S; Obidina, Ju V; Ivanova, Ju S; Zenin, V V; Shatrova, A N; Borodkina, A V; Aksenov, N D; Zemelko, V I; Burova, E B; Puzanov, M V; Nikolsky, N N.
Afiliación
  • Lyublinskaya OG; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
  • Borisov YG; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
  • Pugovkina NA; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
  • Smirnova IS; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
  • Obidina JV; Department of Medical Physics, Institute of Physics, Nanotechnology and Telecommunications, Saint Petersburg State Polytechnic University, Saint Petersburg 195251, Russia.
  • Ivanova JS; Department of Medical Physics, Institute of Physics, Nanotechnology and Telecommunications, Saint Petersburg State Polytechnic University, Saint Petersburg 195251, Russia.
  • Zenin VV; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
  • Shatrova AN; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
  • Borodkina AV; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
  • Aksenov ND; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
  • Zemelko VI; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
  • Burova EB; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
  • Puzanov MV; Federal North-West Medical Research Centre, Saint Petersburg 197341, Russia.
  • Nikolsky NN; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia ; Department of Medical Physics, Institute of Physics, Nanotechnology and Telecommunications, Saint Petersburg State Polytechnic University, Saint Petersburg 19525
Oxid Med Cell Longev ; 2015: 502105, 2015.
Article en En | MEDLINE | ID: mdl-26273423
The present study focuses on the involvement of reactive oxygen species (ROS) in the process of mesenchymal stem cells "waking up" and entering the cell cycle after the quiescence. Using human endometrial mesenchymal stem cells (eMSCs), we showed that intracellular basal ROS level is positively correlated with the proliferative status of the cell cultures. Our experiments with the eMSCs synchronized in the G0 phase of the cell cycle revealed a transient increase in the ROS level upon the quiescence exit after stimulation of the cell proliferation. This increase was registered before the eMSC entry to the S-phase of the cell cycle, and elimination of this increase by antioxidants (N-acetyl-L-cysteine, Tempol, and Resveratrol) blocked G1-S-phase transition. Similarly, a cell cycle arrest which resulted from the antioxidant treatment was observed in the experiments with synchronized human mesenchymal stem cells derived from the adipose tissue. Thus, we showed that physiologically relevant level of ROS is required for the initiation of human mesenchymal stem cell proliferation and that low levels of ROS due to the antioxidant treatment can block the stem cell self-renewal.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Especies Reactivas de Oxígeno / Células Madre Mesenquimatosas Límite: Humans Idioma: En Revista: Oxid Med Cell Longev Asunto de la revista: METABOLISMO Año: 2015 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Especies Reactivas de Oxígeno / Células Madre Mesenquimatosas Límite: Humans Idioma: En Revista: Oxid Med Cell Longev Asunto de la revista: METABOLISMO Año: 2015 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos