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Engineering of a Secretory Active Three-Dimensional Lacrimal Gland Construct on the Basis of Decellularized Lacrimal Gland Tissue.
Spaniol, Kristina; Metzger, Marco; Roth, Mathias; Greve, Burkhard; Mertsch, Sonja; Geerling, Gerd; Schrader, Stefan.
Afiliación
  • Spaniol K; 1 Department of Ophthalmology, University of Düsseldorf , Düsseldorf, Germany .
  • Metzger M; 2 Department of Tissue Engineering and Regenerative Medicine (TERM), University Hospital Würzburg and Translational Center Würzburg "Regenerative Therapies for Oncology and Musculoscelettal Diseases ," Würzburg, Germany .
  • Roth M; 1 Department of Ophthalmology, University of Düsseldorf , Düsseldorf, Germany .
  • Greve B; 3 Department of Radiotherapy, University of Münster , Münster, Germany .
  • Mertsch S; 4 Institute for Experimental Ophthalmology, University of Münster , Münster, Germany .
  • Geerling G; 1 Department of Ophthalmology, University of Düsseldorf , Düsseldorf, Germany .
  • Schrader S; 1 Department of Ophthalmology, University of Düsseldorf , Düsseldorf, Germany .
Tissue Eng Part A ; 21(19-20): 2605-17, 2015 Oct.
Article en En | MEDLINE | ID: mdl-26222647
Lacrimal gland (LG) insufficiency is a main cause for severe dry eye leading to pain, visual impairment, and eventually loss of sight. Engineering of transplantable LG tissue with secretory capacity is a desirable goal. In this study, a three-dimensional decellularized LG (DC-LG) scaffold with preserved LG morphology was generated by treatment with 1% sodium deoxycholate and DNase solution using porcine LG tissue. To address clinical applicability, the primary in vitro culture of secretory active LG cells from a small tissue biopsy of 1.5 mm diameter was introduced and compared with an established isolation method by enzymatic digestion. Cells from both isolation methods depicted an epithelial phenotype, maintained their secretory capacity for up to 30 days, and exhibited progenitor cell capacity as measured by aldehyde dehydrogenase-1 activity, side population assay, and colony-forming units. Cells from passage 0 were reseeded into the DC-LG and secretory active cells migrated into the tissue. The cells resembled an LG-like morphology and the constructs showed secretory activity. These results demonstrate the possibility of engineering a secretory competent, three-dimensional LG construct using LG cells expanded from a small tissue biopsy and DC-LG as a matrix that provides the native structure and physiological niche for these cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Epiteliales Límite: Animals Idioma: En Revista: Tissue Eng Part A Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Epiteliales Límite: Animals Idioma: En Revista: Tissue Eng Part A Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos