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Hyaluronan synthase 3 (HAS3) overexpression downregulates MV3 melanoma cell proliferation, migration and adhesion.
Takabe, Piia; Bart, Geneviève; Ropponen, Antti; Rilla, Kirsi; Tammi, Markku; Tammi, Raija; Pasonen-Seppänen, Sanna.
Afiliación
  • Takabe P; University of Eastern Finland, Institute of Biomedicine, 70211 Kuopio, Finland. Electronic address: piia.takabe@uef.fi.
  • Bart G; University of Eastern Finland, Institute of Biomedicine, 70211 Kuopio, Finland.
  • Ropponen A; University of Eastern Finland, Institute of Clinical Medicine, 70211 Kuopio, Finland.
  • Rilla K; University of Eastern Finland, Institute of Biomedicine, 70211 Kuopio, Finland.
  • Tammi M; University of Eastern Finland, Institute of Biomedicine, 70211 Kuopio, Finland.
  • Tammi R; University of Eastern Finland, Institute of Biomedicine, 70211 Kuopio, Finland.
  • Pasonen-Seppänen S; University of Eastern Finland, Institute of Biomedicine, 70211 Kuopio, Finland.
Exp Cell Res ; 337(1): 1-15, 2015 Sep 10.
Article en En | MEDLINE | ID: mdl-26222208
Malignant skin melanoma is one of the most deadly human cancers. Extracellular matrix (ECM) influences the growth of malignant tumors by modulating tumor cells adhesion and migration. Hyaluronan is an essential component of the ECM, and its amount is altered in many tumors, suggesting an important role for hyaluronan in tumorigenesis. Nonetheless its role in melanomagenesis is not understood. In this study we produced a MV3 melanoma cell line with inducible expression of the hyaluronan synthase 3 (HAS3) and studied its effect on the behavior of the melanoma cells. HAS3 overexpression expanded the cell surface hyaluronan coat and decreased melanoma cell adhesion, migration and proliferation by cell cycle arrest at G1/G0. Melanoma cell migration was restored by removal of cell surface hyaluronan by Streptomyces hyaluronidase and by receptor blocking with hyaluronan oligosaccharides, while the effect on cell proliferation was receptor independent. Overexpression of HAS3 decreased ERK1/2 phosphorylation suggesting that inhibition of MAP-kinase signaling was responsible for these suppressive effects on the malignant phenotype of MV3 melanoma cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adhesión Celular / Movimiento Celular / Glucuronosiltransferasa / Proliferación Celular / Melanoma Límite: Humans Idioma: En Revista: Exp Cell Res Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adhesión Celular / Movimiento Celular / Glucuronosiltransferasa / Proliferación Celular / Melanoma Límite: Humans Idioma: En Revista: Exp Cell Res Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos