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Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification.
Kruger, Ernesto Renato; Penha, Tania Regina; Stoffelo, Daura Regina Eira; Roehe, Paulo Michel; Ribeiro, Magda Costa; Soccol, Vanete Thomaz.
Afiliación
  • Kruger ER; Centro de Diagnóstico Marcos Enrietti, Curitiba, PR, Brazil. ; Programa de Pós Graduação em Processos Biotecnológicos, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Penha TR; Instituto de Tecnologia do Paraná, Curitiba, PR, Brazil. ; Programa de Pós Graduação em Processos Biotecnológicos, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Stoffelo DR; Centro de Microscopia Eletrônica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Roehe PM; Departamento de Microbiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Ribeiro MC; Departamento de Patologia Básica, Centro Politécnico, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Soccol VT; Programa de Pós Graduação em Processos Biotecnológicos, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Braz J Microbiol ; 46(1): 279-83, 2015 Mar.
Article en En | MEDLINE | ID: mdl-26221118
Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinae sub-family and belongs to genus Rhadinovirus . This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow's uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical capsid symmetry surrounded by a loose envelope, typical of a herpesvirus. A 2,571 bp PCR product after Hind III digestion generated four fragments, whose base pair composition were 403, 420, 535, and 1,125 bp. Restriction enzymes Hind III and Bam HI generated the expected diagnostic bands as well as a 2,350 bp hypermolar fragment as a result of Bam HI treatment to demonstrate that agent was a bovine herpesvirus 4, appertaining to DN-599 group.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Tumorales por Virus / Enfermedades de los Bovinos / Infecciones por Herpesviridae / Herpesvirus Bovino 4 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Braz J Microbiol Año: 2015 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Tumorales por Virus / Enfermedades de los Bovinos / Infecciones por Herpesviridae / Herpesvirus Bovino 4 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Braz J Microbiol Año: 2015 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil