Transcriptional coordination of physiological responses in Nannochloropsis oceanica CCMP1779 under light/dark cycles.

Poliner, Eric; Panchy, Nicholas; Newton, Linsey; Wu, Guangxi; Lapinsky, Andrew; Bullard, Blair; Zienkiewicz, Agnieszka; Benning, Christoph; Shiu, Shin-Han; Farré, Eva M

Poliner, Eric; Panchy, Nicholas; Newton, Linsey; Wu, Guangxi; Lapinsky, Andrew; Bullard, Blair; Zienkiewicz, Agnieszka; Benning, Christoph; Shiu, Shin-Han; Farré, Eva M.

Afiliación

Poliner E; Cell and Molecular Biology Program, Michigan State University, East Lansing, MI, 48824, USA.
Panchy N; Genetics Program, Michigan State University, East Lansing, MI, 48824, USA.
Newton L; Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.
Wu G; Cell and Molecular Biology Program, Michigan State University, East Lansing, MI, 48824, USA.
Lapinsky A; Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.
Bullard B; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824, USA.
Zienkiewicz A; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824, USA.
Benning C; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824, USA.
Shiu SH; Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.
Farré EM; Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.

Plant J ; 83(6): 1097-113, 2015 Sep.

Article en En | MEDLINE | ID: mdl-26216534

RESUMEN

Nannochloropsis oceanica CCMP1779 is a marine unicellular stramenopile and an emerging reference species for basic research on oleogenic microalgae with biotechnological relevance. We investigated its physiology and transcriptome under light/dark cycles. We observed oscillations in lipid content and a predominance of cell division in the first half of the dark phase. Globally, more than 60% of the genes cycled in N. oceanica CCMP1779, with gene expression peaking at different times of the day. Interestingly, the phase of expression of genes involved in certain biological processes was conserved across photosynthetic lineages. Furthermore, in agreement with our physiological studies we found the processes of lipid metabolism and cell division enriched in cycling genes. For example, there was tight coordination of genes involved in the lower part of glycolysis, fatty acid synthesis and lipid production at dawn preceding lipid accumulation during the day. Our results suggest that diel lipid storage plays a key role for N. oceanica CCMP1779 growth under natural conditions making this alga a promising model to gain a basic mechanistic understanding of triacylglycerol production in photosynthetic cells. Our data will help the formulation of new hypotheses on the role of cyclic gene expression in cell growth and metabolism in Nannochloropsis.

Asunto(s)

Regulación de la Expresión Génica; Estramenopilos/fisiología; Acetilcoenzima A/metabolismo; Carbono/metabolismo; Ciclo Celular/genética; Ciclo del Ácido Cítrico/fisiología; Ácidos Grasos/genética; Ácidos Grasos/metabolismo; Glucólisis; Metabolismo de los Lípidos/genética; Fotoperiodo; Estramenopilos/genética; Estramenopilos/metabolismo

Palabras clave

Nannochloropsis oceanica; cell cycle; diel; lipids; metabolism; transcriptome

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación de la Expresión Génica / Estramenopilos Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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