Your browser doesn't support javascript.
loading
Transient receptor potential vanilloid 2-mediated shear-stress responses in C2C12 myoblasts are regulated by serum and extracellular matrix.
Kurth, Felix; Franco-Obregón, Alfredo; Casarosa, Marco; Küster, Simon K; Wuertz-Kozak, Karin; Dittrich, Petra S.
Afiliación
  • Kurth F; *Department of Biosystems and Science Engineering and Institute for Biomechanics, Eidgenössische Technische Hochschule Zürich, Switzerland; Department of Surgery, Yong Loo Lin School of Medicine, and Department of Physiology, National University of Singapore, Singapore; and National University Hospi
  • Franco-Obregón A; *Department of Biosystems and Science Engineering and Institute for Biomechanics, Eidgenössische Technische Hochschule Zürich, Switzerland; Department of Surgery, Yong Loo Lin School of Medicine, and Department of Physiology, National University of Singapore, Singapore; and National University Hospi
  • Casarosa M; *Department of Biosystems and Science Engineering and Institute for Biomechanics, Eidgenössische Technische Hochschule Zürich, Switzerland; Department of Surgery, Yong Loo Lin School of Medicine, and Department of Physiology, National University of Singapore, Singapore; and National University Hospi
  • Küster SK; *Department of Biosystems and Science Engineering and Institute for Biomechanics, Eidgenössische Technische Hochschule Zürich, Switzerland; Department of Surgery, Yong Loo Lin School of Medicine, and Department of Physiology, National University of Singapore, Singapore; and National University Hospi
  • Wuertz-Kozak K; *Department of Biosystems and Science Engineering and Institute for Biomechanics, Eidgenössische Technische Hochschule Zürich, Switzerland; Department of Surgery, Yong Loo Lin School of Medicine, and Department of Physiology, National University of Singapore, Singapore; and National University Hospi
  • Dittrich PS; *Department of Biosystems and Science Engineering and Institute for Biomechanics, Eidgenössische Technische Hochschule Zürich, Switzerland; Department of Surgery, Yong Loo Lin School of Medicine, and Department of Physiology, National University of Singapore, Singapore; and National University Hospi
FASEB J ; 29(11): 4726-37, 2015 Nov.
Article en En | MEDLINE | ID: mdl-26207028
The developmental sensitivity of skeletal muscle to mechanical forces is unparalleled in other tissues. Calcium entry via reputedly mechanosensitive transient receptor potential (TRP) channel classes has been shown to play an essential role in both the early proliferative stage and subsequent differentiation of skeletal muscle myoblasts, particularly TRP canonical (TRPC) 1 and TRP vanilloid (TRPV) 2. Here we show that C2C12 murine myoblasts respond to fluid flow-induced shear stress with increments in cytosolic calcium that are largely initiated by the mechanosensitive opening of TRPV2 channels. Response to fluid flow was augmented by growth in low extracellular serum concentration (5 vs. 20% fetal bovine serum) by greater than 9-fold and at 18 h in culture, coincident with the greatest TRPV2 channel expression under identical conditions (P < 0.02). Fluid flow responses were also enhanced by substrate functionalization with laminin, rather than with fibronectin, agreeing with previous findings that the gating of TRPV2 is facilitated by laminin. Fluid flow-induced calcium increments were blocked by ruthenium red (27%) and SKF-96365 (38%), whereas they were unaltered by 2-aminoethoxydiphenyl borate, further corroborating that TRPV2 channels play a predominant role in fluid flow mechanosensitivity over that of TRPC1 and TRP melastatin (TRPM) 7.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Canales de Calcio / Activación del Canal Iónico / Mioblastos / Mecanotransducción Celular / Canales Catiónicos TRPV / Matriz Extracelular Límite: Animals Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Canales de Calcio / Activación del Canal Iónico / Mioblastos / Mecanotransducción Celular / Canales Catiónicos TRPV / Matriz Extracelular Límite: Animals Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos