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Inhibition of Schistosoma mansoni ether-a-go-go related gene-encoded potassium channels leads to hypermotility and impaired egg production.
Parker-Manuel, S J; Hahnel, S; Grevelding, C G.
Afiliación
  • Parker-Manuel SJ; Institute of Parasitology, Justus-Liebig-University Giessen, Germany. Electronic address: sophie_manuel@yahoo.co.uk.
  • Hahnel S; Institute of Parasitology, Justus-Liebig-University Giessen, Germany.
  • Grevelding CG; Institute of Parasitology, Justus-Liebig-University Giessen, Germany.
Exp Parasitol ; 158: 48-54, 2015 Nov.
Article en En | MEDLINE | ID: mdl-26188142
The purpose of this work was to investigate the effect of ether-a-go-go related gene (ERG) potassium channel inhibition on Schistosoma mansoni. Use of dofetilide to block the schistosome ERGs resulted in a striking 'corkscrew' effect. The worms were unable to control their motility; they were hypermotile. The treated worms produced abnormal eggs, some of which consisted of little more than a spine. One of the S. mansoni ERGs (SmERGs), Smp_161140, was chosen for further study by RNAi. The transcript was knocked down to 50% compared to the controls. These RNAi-treated worms demonstrated seizure-like movements. In S. mansoni, as in other organisms, ERG channels seem to play a role in regulating muscle excitability. This work shows that egg production can be greatly reduced by effectively targeting muscle coordination in these important parasites.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Schistosoma mansoni / Canales de Potasio Éter-A-Go-Go Límite: Animals Idioma: En Revista: Exp Parasitol Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Schistosoma mansoni / Canales de Potasio Éter-A-Go-Go Límite: Animals Idioma: En Revista: Exp Parasitol Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos