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Evaluation of the effect of elevated intraocular pressure and reduced ocular perfusion pressure on retinal capillary bed filling and total retinal blood flow in rats by OMAG/OCT.
Zhi, Zhongwei; Cepurna, William; Johnson, Elaine; Jayaram, Hari; Morrison, John; Wang, Ruikang K.
Afiliación
  • Zhi Z; Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
  • Cepurna W; Casey Eye Institute, Oregon Health & Science University, Portland, OR 97239, USA.
  • Johnson E; Casey Eye Institute, Oregon Health & Science University, Portland, OR 97239, USA.
  • Jayaram H; Casey Eye Institute, Oregon Health & Science University, Portland, OR 97239, USA.
  • Morrison J; Casey Eye Institute, Oregon Health & Science University, Portland, OR 97239, USA.
  • Wang RK; Department of Bioengineering, University of Washington, Seattle, WA 98195, USA; Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA. Electronic address: wangrk@uw.edu.
Microvasc Res ; 101: 86-95, 2015 Sep.
Article en En | MEDLINE | ID: mdl-26186381
PURPOSE: To determine if retinal capillary filling is preserved in the face of acutely elevated intraocular pressure (IOP) in anesthetized rats, despite a reduction in total retinal blood flow (RBF), using optical microangiography/optical coherence tomography (OMAG/OCT). METHODS: OMAG provided the capability of depth-resolved imaging of the retinal microvasculature down to the capillary level. Doppler OCT was applied to measure the total RBF using an enface integration approach. The microvascular pattern, capillary density, and total RBF were monitored in vivo as the IOP was increased from 10 to 100mmHg in 10mmHg intervals and returned back to 10mmHg. RESULTS: In animals with mean arterial pressure (MAP) of 102±4mmHg (n=10), when IOP was increased from 0 to 100mmHg, the capillary density remained at or above 80% of baseline for the IOP up to 60mmHg [or ocular perfusion pressure (OPP) at 40mmHg]. This was then decreased, achieving 60% of baseline at IOP 70mmHg and OPP of 30mmHg. Total RBF was unaffected by moderate increases in IOP up to 30mmHg, beyond which total RBF decreased linearly, reaching 50% of baseline at IOP 60mmHg and OPP 40mmHg. Both capillary density and total RBF were totally extinguished at 100mmHg, but fully recovered when IOP returned to baseline. By comparison, a separate group of animals with lower MAP (mean=75±6mmHg, n=7) demonstrated comparable decreases in both capillary filling and total RBF at IOPs that were 20mmHg lower than in the initial group. Both were totally extinguished at 80mmHg, but fully recovered when IOP returned to baseline. Relationships of both parameters to OPP were unchanged. CONCLUSION: Retinal capillary filling and total RBF responses to IOP elevation can be monitored non-invasively by OMAG/OCT and both are influenced by OPP. Retinal capillary filling was relatively preserved down to a perfusion pressure of 40mmHg, despite a linear reduction in total RBF.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Flujo Sanguíneo Regional / Retina / Vasos Retinianos / Presión Intraocular Límite: Animals Idioma: En Revista: Microvasc Res Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Flujo Sanguíneo Regional / Retina / Vasos Retinianos / Presión Intraocular Límite: Animals Idioma: En Revista: Microvasc Res Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos