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New filtration system for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts.
Al-Sabi, M N S; Gad, J A; Riber, U; Kurtzhals, J A L; Enemark, H L.
Afiliación
  • Al-Sabi MN; Section for Bacteriology, Pathology and Parasitology, National Veterinary Institute, Technical University of Denmark, Frederiksberg C, Denmark.
  • Gad JA; Centre for Medical Parasitology, Department of International Health, Immunology, and Microbiology, University of Copenhagen, Copenhagen, Denmark.
  • Riber U; Department of Clinical Microbiology, Copenhagen University Hospital, Copenhagen, Denmark.
  • Kurtzhals JA; Grundfos Management A/S, Bjerringbro, Denmark.
  • Enemark HL; Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Frederiksberg C, Denmark.
J Appl Microbiol ; 119(3): 894-903, 2015 Sep.
Article en En | MEDLINE | ID: mdl-26172033
AIMS: To develop a filtration unit for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts ((oo-)cysts) in drinking water. METHODS AND RESULTS: This unit utilizes a metallic filter and an ultrasound transducer for eluting (oo-)cysts, with a fixed retentate backwash volume; approx. 400 µl. Changes in the viability was evaluated by seeding wild type (oo-)cysts (1 × 10(4)) followed by sonication for 5, 10, 20 or 40 s (five replicates for each period). Flow cytometry analysis showed negligible increase in the mortality of (oo-)cysts exposed to 5-10 s of sonication. Recovery rate was assessed by seeding ColorSeed(™) (10 replicates) into the filter unit followed by air backwash to a glass slide and counting of (oo-)cysts by epifluorescent microscopy. High recovery rates (mean ± SD) were found: 84·9% ± 4·8 for Giardia cysts and 70% ± 6·5 for Cryptosporidium oocysts. DNA of seeded wild type (oo-)cysts (1 × 10(2); 10 replicates) was successfully amplified using real-time PCR. CONCLUSIONS: The use of a metallic filter, sonication and 'air backwash' were key factors for creating a highly efficient system for recovery of apparently undamaged protozoa. SIGNIFICANCE AND IMPACT OF THE STUDY: This reagent-less system can be used for monitoring of parasite contamination in drinking water.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua Potable / Purificación del Agua / Cryptosporidium / Filtración / Giardia Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua Potable / Purificación del Agua / Cryptosporidium / Filtración / Giardia Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido