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Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa.
Sánchez-Calabuig, M J; de la Fuente, J; Laguna-Barraza, R; Beltrán-Breña, P; Martínez-Nevado, E; Johnston, S D; Rizos, D; Gutiérrez-Adán, A; Pérez-Gutiérrez, J F.
Afiliación
  • Sánchez-Calabuig MJ; Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
  • de la Fuente J; Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
  • Laguna-Barraza R; Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
  • Beltrán-Breña P; Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
  • Martínez-Nevado E; Zoo-Aquarium, Madrid, Spain.
  • Johnston SD; Wildlife Science Unit, School of Agriculture and Food Science, University of Queensland, Gatton, Australia.
  • Rizos D; Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
  • Gutiérrez-Adán A; Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
  • Pérez-Gutiérrez JF; Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, Madrid, Spain. Electronic address: jfperez@ucm.es.
Theriogenology ; 84(6): 983-94, 2015 Oct 01.
Article en En | MEDLINE | ID: mdl-26149074
Assisted reproductive technologies are of great importance for increasing the genetic diversity in captive animals. The use of bovine or murine oocytes in heterologous IVF provides advantages compared to homologous IVF in nondomestic animals, such as the accessibility to oocytes and the availability of well-developed in vitro maturation systems. The aim of this study was to determine the heterologous IVF parameters using cryopreserved dolphin spermatozoa and zona-intact bovine or murine oocytes and to examine the nuclear chromatin status of the dolphin spermatozoa. All the processes involved in the fertilization including embryo cleavage were observed by confocal microscopy and hybrid embryo formation was confirmed by polymerase chain reaction. Heterologous bovine IVF showed no polyspermy, lower percentages of pronuclear formation, and a lower cleavage rate compared to homologous IVF group (34.8% vs. 89.3%). Heterologous murine IVF showed a lower cleavage rate than homologous IVF (9.6% vs. 77.1%). With respect to dolphin sperm chromatin, it was more stable, i.e. more resistant to EDTA-SDS decondensation than the bovine sperm chromatin. This study revealed the stability of the dolphin sperm chromatin and the ability of the dolphin spermatozoa to penetrate zona-intact bovine and murine oocytes, leading to hybrid embryo formation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fertilización In Vitro / Delfín Mular / Fertilización Límite: Animals Idioma: En Revista: Theriogenology Año: 2015 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fertilización In Vitro / Delfín Mular / Fertilización Límite: Animals Idioma: En Revista: Theriogenology Año: 2015 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos