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Unidirectional incorporation of a bacterial mechanosensitive channel into liposomal membranes.
Nomura, Takeshi; Cox, Charles D; Bavi, Navid; Sokabe, Masahiro; Martinac, Boris.
Afiliación
  • Nomura T; *Molecular Cardiology and Biophysics Division/Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia; Department of Physical Therapy, Faculty of Rehabilitation, Graduate School of Health Sciences, Kyushu Nutrition Welfare University, K
  • Cox CD; *Molecular Cardiology and Biophysics Division/Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia; Department of Physical Therapy, Faculty of Rehabilitation, Graduate School of Health Sciences, Kyushu Nutrition Welfare University, K
  • Bavi N; *Molecular Cardiology and Biophysics Division/Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia; Department of Physical Therapy, Faculty of Rehabilitation, Graduate School of Health Sciences, Kyushu Nutrition Welfare University, K
  • Sokabe M; *Molecular Cardiology and Biophysics Division/Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia; Department of Physical Therapy, Faculty of Rehabilitation, Graduate School of Health Sciences, Kyushu Nutrition Welfare University, K
  • Martinac B; *Molecular Cardiology and Biophysics Division/Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia; Department of Physical Therapy, Faculty of Rehabilitation, Graduate School of Health Sciences, Kyushu Nutrition Welfare University, K
FASEB J ; 29(10): 4334-45, 2015 Oct.
Article en En | MEDLINE | ID: mdl-26116700
The bacterial mechanosensitive channel of small conductance (MscS) plays a crucial role in the protection of bacterial cells against hypo-osmotic shock. The functional characteristics of MscS have been extensively studied using liposomal reconstitution. This is a widely used experimental paradigm and is particularly important for mechanosensitive channels as channel activity can be probed free from cytoskeletal influence. A perpetual issue encountered using this paradigm is unknown channel orientation. Here we examine the orientation of MscS in liposomes formed using 2 ion channel reconstitution methods employing the powerful combination of patch clamp electrophysiology, confocal microscopy, and continuum mechanics simulation. Using the previously determined electrophysiological and pharmacological properties of MscS, we were able to determine that in liposomes, independent of lipid composition, MscS adopts the same orientation seen in native membranes. These results strongly support the idea that these specific methods result in uniform incorporation of membrane ion channels and caution against making assumptions about mechanosensitive channel orientation using the stimulus type alone.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación del Canal Iónico / Proteínas de Escherichia coli / Mecanotransducción Celular / Canales Iónicos Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación del Canal Iónico / Proteínas de Escherichia coli / Mecanotransducción Celular / Canales Iónicos Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos