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Differential expression of id genes and their potential regulator znf238 in zebrafish adult neural progenitor cells and neurons suggests distinct functions in adult neurogenesis.
Diotel, Nicolas; Beil, Tanja; Strähle, Uwe; Rastegar, Sepand.
Afiliación
  • Diotel N; Karlsruhe Institute of Technology, Campus Nord, Institute of Toxicology and Genetics, Karlsruhe, Germany; Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), Plateforme CYROI, Sainte-Clotilde, F-97490, France; Université de La Réunion, UMR 1188, Sainte-Clotilde, F-97490
  • Beil T; Karlsruhe Institute of Technology, Campus Nord, Institute of Toxicology and Genetics, Karlsruhe, Germany.
  • Strähle U; Karlsruhe Institute of Technology, Campus Nord, Institute of Toxicology and Genetics, Karlsruhe, Germany.
  • Rastegar S; Karlsruhe Institute of Technology, Campus Nord, Institute of Toxicology and Genetics, Karlsruhe, Germany. Electronic address: Sepand.Rastegar@kit.edu.
Gene Expr Patterns ; 19(1-2): 1-13, 2015.
Article en En | MEDLINE | ID: mdl-26107416
Teleost fish display a remarkable ability to generate new neurons and to repair brain lesions during adulthood. They are, therefore, a very popular model to investigate the molecular mechanisms of constitutive and induced neurogenesis in adult vertebrates. In this study, we investigated the expression patterns of inhibitor of DNA binding (id) genes and of their potential transcriptional repressor, znf238, in the whole brain of adult zebrafish. We show that while id1 is exclusively expressed in ventricular cells in the whole brain, id2a, id3 and id4 genes are expressed in broader areas. Interestingly, znf238 was also detected in these regions, its expression overlapping with id2a, id3 and id4 expression. Further detailed characterization of the id-expressing cells demonstrated that (a) id1 is expressed in type 1 and type 2 neural progenitors as previously published, (b) id2a in type 1, 2 and 3 neural progenitors, (c) id3 in type 3 neural progenitors and (d) id4 in postmitotic neurons. Our data provide a detailed map of id and znf238 expression in the brain of adult zebrafish, supplying a framework for studies of id genes function during adult neurogenesis and brain regeneration in the zebrafish.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Represoras / Pez Cebra / Proteínas Inhibidoras de la Diferenciación / Neurogénesis / Células-Madre Neurales / Neuronas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Gene Expr Patterns Asunto de la revista: BIOLOGIA MOLECULAR Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Represoras / Pez Cebra / Proteínas Inhibidoras de la Diferenciación / Neurogénesis / Células-Madre Neurales / Neuronas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Gene Expr Patterns Asunto de la revista: BIOLOGIA MOLECULAR Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos