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Nanoscale alterations of corneocytes indicate skin disease.
Franz, J; Beutel, M; Gevers, K; Kramer, A; Thyssen, J P; Kezic, S; Riethmüller, C.
Afiliación
  • Franz J; nAnostic Institute, Centre for Nanotechnology, University of Münster, Münster, Germany.
  • Beutel M; nAnostic Institute, Centre for Nanotechnology, University of Münster, Münster, Germany.
  • Gevers K; Institute of Astrophysics, University of Heidelberg, Heidelberg, Germany.
  • Kramer A; nAnostic Institute, Centre for Nanotechnology, University of Münster, Münster, Germany.
  • Thyssen JP; Serend-ip GmbH, Centre for Nanotechnology, Münster, Germany.
  • Kezic S; Dept. Dermatology and Allergology, Gentofte University Hospital, Copenhagen, Denmark.
  • Riethmüller C; Coronel Institute, AMC, University of Amsterdam, Amsterdam, The Netherlands.
Skin Res Technol ; 22(2): 174-80, 2016 May.
Article en En | MEDLINE | ID: mdl-26100642
BACKGROUND: The skin barrier protects the organism against exogenous stressors and simultaneously prevents excessive water loss. While the delicate regulation of skin barrier is not completely understood, morphological and histological evaluation remain key features of clinical investigations. Here, we extended the phenotypic perspective down to the nanoscale. METHODS: Corneocyte samples were obtained non-invasively by a standard tape stripping procedure from 21 indviduals. Scanning electron (SEM) and atomic force microcopy (AFM) were used to record nanoscale topography. Circular nano-objects were identified and these were quantitated through computer vision. RESULTS: Typical dimensions of 273 nm height and 305 nm width. We showed that their density does not correlate to age or pigmentation in healthy subjects, but that they were clearly elevated in corneocytes from patients with atopic dermatitis, a common inflammatory skin condition. CONCLUSION: The presence of these corneocyte-nanostructures might be used as a diagnostic parameter for skin disorders - even in cases below a clinical threshold.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Queratinocitos / Dermatitis Atópica Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Revista: Skin Res Technol Asunto de la revista: DERMATOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Queratinocitos / Dermatitis Atópica Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Revista: Skin Res Technol Asunto de la revista: DERMATOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido