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Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro.
Taketa, Hiroaki; Sathi, Gulsan Ara; Farahat, Mahmoud; Rahman, Kazi Anisur; Sakai, Takayoshi; Hirano, Yoshiaki; Kuboki, Takuo; Torii, Yasuhiro; Matsumoto, Takuya.
Afiliación
  • Taketa H; Department of Biomaterials, Okayama University, 2-5-1 Shikata-Cho, Okayama, 700-8558, Japan.
  • Sathi GA; Department of Biomaterials, Okayama University, 2-5-1 Shikata-Cho, Okayama, 700-8558, Japan.
  • Farahat M; Department of Biomaterials, Okayama University, 2-5-1 Shikata-Cho, Okayama, 700-8558, Japan.
  • Rahman KA; Department of Biomaterials, Okayama University, 2-5-1 Shikata-Cho, Okayama, 700-8558, Japan.
  • Sakai T; Department of Oral-Facial Disorders, Osaka University, 1-8 Yamada-oka, Suita, 565-0871, Japan.
  • Hirano Y; Department of Chemical Engineering, Kansai University, 3-3-1 Yamate, Suita 564-8680, Japan.
  • Kuboki T; Department of Biomaterials, Okayama University, 2-5-1 Shikata-Cho, Okayama, 700-8558, Japan.
  • Torii Y; Department of Biomaterials, Okayama University, 2-5-1 Shikata-Cho, Okayama, 700-8558, Japan.
  • Matsumoto T; Department of Biomaterials, Okayama University, 2-5-1 Shikata-Cho, Okayama, 700-8558, Japan.
Sci Rep ; 5: 11468, 2015 Jun 22.
Article en En | MEDLINE | ID: mdl-26098225
In vitro fabricated biological tissue would be a valuable tool to screen newly synthesized drugs or understand the tissue development process. Several studies have attempted to fabricate biological tissue in vitro. However, controlling the growth and morphology of the fabricated tissue remains a challenge. Therefore, new techniques are required to modulate tissue growth. RGD (arginine-glycine-aspartic acid), which is an integrin-binding domain of fibronectin, has been found to enhance cell adhesion and survival; it has been used to modify substrates for in vitro cell culture studies or used as tissue engineering scaffolds. In addition, this study shows novel functions of the RGD peptide, which enhances tissue growth and modulates tissue morphology in vitro. When an isolated submandibular gland (SMG) was cultured on an RGD-modified alginate hydrogel sheet, SMG growth including bud expansion and cleft formation was dramatically enhanced. Furthermore, we prepared small RGD-modified alginate beads and placed them on the growing SMG tissue. These RGD-modified beads successfully induced cleft formation at the bead position, guiding the desired SMG morphology. Thus, this RGD-modified material might be a promising tool to modulate tissue growth and morphology in vitro for biological tissue fabrication.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligopéptidos / Glándula Submandibular / Ingeniería de Tejidos Límite: Animals Idioma: En Revista: Sci Rep Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligopéptidos / Glándula Submandibular / Ingeniería de Tejidos Límite: Animals Idioma: En Revista: Sci Rep Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido