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Dengue virus NS1 protein interacts with the ribosomal protein RPL18: this interaction is required for viral translation and replication in Huh-7 cells.
Cervantes-Salazar, Margot; Angel-Ambrocio, Antonio H; Soto-Acosta, Ruben; Bautista-Carbajal, Patricia; Hurtado-Monzon, Arianna M; Alcaraz-Estrada, Sofia L; Ludert, Juan E; Del Angel, Rosa M.
Afiliación
  • Cervantes-Salazar M; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico DF, Mexico.
  • Angel-Ambrocio AH; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico DF, Mexico.
  • Soto-Acosta R; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico DF, Mexico.
  • Bautista-Carbajal P; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico DF, Mexico.
  • Hurtado-Monzon AM; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico DF, Mexico.
  • Alcaraz-Estrada SL; National Medical Center November 20, (ISSSTE), Mexico DF, Mexico.
  • Ludert JE; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico DF, Mexico.
  • Del Angel RM; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico DF, Mexico. Electronic address: rmangel@cinvestav.mx.
Virology ; 484: 113-126, 2015 Oct.
Article en En | MEDLINE | ID: mdl-26092250
Given dengue virus (DENV) genome austerity, it uses cellular molecules and structures for virion entry, translation and replication of the genome. NS1 is a multifunctional protein key to viral replication and pathogenesis. Identification of cellular proteins that interact with NS1 may help in further understanding the functions of NS1. In this paper we isolated a total of 64 proteins from DENV infected human hepatic cells (Huh-7) that interact with NS1 by affinity chromatography and immunoprecipitation assays. The subcellular location and expression levels during infection of the ribosomal proteins RPS3a, RPL7, RPL18, RPL18a plus GAPDH were determined. None of these proteins changed their expression levels during infection; however, RPL-18 was redistributed to the perinuclear region after 48hpi. Silencing of the RPL-18 does not affect cell translation efficiency or viability, but it reduces significantly viral translation, replication and viral yield, suggesting that the RPL-18 is required during DENV replicative cycle.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Ribosómicas / Biosíntesis de Proteínas / Replicación Viral / Proteínas no Estructurales Virales / Hepatocitos / Virus del Dengue / Interacciones Huésped-Patógeno Límite: Humans Idioma: En Revista: Virology Año: 2015 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Ribosómicas / Biosíntesis de Proteínas / Replicación Viral / Proteínas no Estructurales Virales / Hepatocitos / Virus del Dengue / Interacciones Huésped-Patógeno Límite: Humans Idioma: En Revista: Virology Año: 2015 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos