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Collembolan Transcriptomes Highlight Molecular Evolution of Hexapods and Provide Clues on the Adaptation to Terrestrial Life.
Faddeeva, A; Studer, R A; Kraaijeveld, K; Sie, D; Ylstra, B; Mariën, J; op den Camp, H J M; Datema, E; den Dunnen, J T; van Straalen, N M; Roelofs, D.
Afiliación
  • Faddeeva A; Department of Ecological Science, VU University Amsterdam, Amsterdam, The Netherlands.
  • Studer RA; EMBL-European Bioinformatics Institute, Cambridge, United Kingdom.
  • Kraaijeveld K; Department of Ecological Science, VU University Amsterdam, Amsterdam, The Netherlands.
  • Sie D; Microarray Facility, VU Medical Center, Amsterdam, The Netherlands.
  • Ylstra B; Microarray Facility, VU Medical Center, Amsterdam, The Netherlands.
  • Mariën J; Department of Ecological Science, VU University Amsterdam, Amsterdam, The Netherlands.
  • op den Camp HJ; Department of Microbiology, Radboud University Nijmegen, Nijmegen, The Netherlands.
  • Datema E; Keygene NV, Wageningen, The Netherlands.
  • den Dunnen JT; Leiden Genome Technology Center, Human and Clinical Genetics, Leiden University Medical Center, Leiden, The Netherlands.
  • van Straalen NM; Department of Ecological Science, VU University Amsterdam, Amsterdam, The Netherlands.
  • Roelofs D; Department of Ecological Science, VU University Amsterdam, Amsterdam, The Netherlands.
PLoS One ; 10(6): e0130600, 2015.
Article en En | MEDLINE | ID: mdl-26075903
BACKGROUND: Collembola (springtails) represent a soil-living lineage of hexapods in between insects and crustaceans. Consequently, their genomes may hold key information on the early processes leading to evolution of Hexapoda from a crustacean ancestor. METHOD: We assembled and annotated transcriptomes of the Collembola Folsomia candida and Orchesella cincta, and performed comparative analysis with protein-coding gene sequences of three crustaceans and three insects to identify adaptive signatures associated with the evolution of hexapods within the pancrustacean clade. RESULTS: Assembly of the springtail transcriptomes resulted in 37,730 transcripts with predicted open reading frames for F. candida and 32,154 for O. cincta, of which 34.2% were functionally annotated for F. candida and 38.4% for O. cincta. Subsequently, we predicted orthologous clusters among eight species and applied the branch-site test to detect episodic positive selection in the Hexapoda and Collembola lineages. A subset of 250 genes showed significant positive selection along the Hexapoda branch and 57 in the Collembola lineage. Gene Ontology categories enriched in these genes include metabolism, stress response (i.e. DNA repair, immune response), ion transport, ATP metabolism, regulation and development-related processes (i.e. eye development, neurological development). CONCLUSIONS: We suggest that the identified gene families represent processes that have played a key role in the divergence of hexapods within the pancrustacean clade that eventually evolved into the most species-rich group of all animals, the hexapods. Furthermore, some adaptive signatures in collembolans may provide valuable clues to understand evolution of hexapods on land.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artrópodos Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2015 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artrópodos Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2015 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos