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Structure determination of an integral membrane protein at room temperature from crystals in situ.
Axford, Danny; Foadi, James; Hu, Nien Jen; Choudhury, Hassanul Ghani; Iwata, So; Beis, Konstantinos; Evans, Gwyndaf; Alguel, Yilmaz.
Afiliación
  • Axford D; Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, England.
  • Foadi J; Membrane Protein Laboratory, Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, England.
  • Hu NJ; Membrane Protein Laboratory, Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, England.
  • Choudhury HG; Membrane Protein Laboratory, Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, England.
  • Iwata S; Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, England.
  • Beis K; Membrane Protein Laboratory, Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, England.
  • Evans G; Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, England.
  • Alguel Y; Membrane Protein Laboratory, Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, England.
Acta Crystallogr D Biol Crystallogr ; 71(Pt 6): 1228-37, 2015 Jun.
Article en En | MEDLINE | ID: mdl-26057664
The structure determination of an integral membrane protein using synchrotron X-ray diffraction data collected at room temperature directly in vapour-diffusion crystallization plates (in situ) is demonstrated. Exposing the crystals in situ eliminates manual sample handling and, since it is performed at room temperature, removes the complication of cryoprotection and potential structural anomalies induced by sample cryocooling. Essential to the method is the ability to limit radiation damage by recording a small amount of data per sample from many samples and subsequently assembling the resulting data sets using specialized software. The validity of this procedure is established by the structure determination of Haemophilus influenza TehA at 2.3 Šresolution. The method presented offers an effective protocol for the fast and efficient determination of membrane-protein structures at room temperature using third-generation synchrotron beamlines.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cristalografía por Rayos X / Proteínas de la Membrana Idioma: En Revista: Acta Crystallogr D Biol Crystallogr Año: 2015 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cristalografía por Rayos X / Proteínas de la Membrana Idioma: En Revista: Acta Crystallogr D Biol Crystallogr Año: 2015 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos