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Enhanced photocurrent from Photosystem I upon in vitro truncation of the antennae chlorophyll.
Carter, J Ridge; Baker, David R; Witt, T Austin; Bruce, Barry D.
Afiliación
  • Carter JR; Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, 37996, USA.
  • Baker DR; Sensors and Electron Devices Directorate, United States Army Research Laboratory, Adelphi, MD, 20783, USA.
  • Witt TA; Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, 37996, USA.
  • Bruce BD; Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, 37996, USA. bbruce@utk.edu.
Photosynth Res ; 127(2): 161-70, 2016 Feb.
Article en En | MEDLINE | ID: mdl-26031418
Current effects on climate change and dwindling fossil fuel reserves require new materials and methods to convert solar energy into a viable clean energy source. Recent progress in the direct conversion of light into photocurrent has been well documented using Photosystem I. In plants, PSI consists of a core complex and multiple light-harvesting complexes, denoted LHCI and LHCII. Most of the methods for isolating PSI from plants involve a selective, detergent solubilization from thylakoids followed by sucrose gradient density centrifugation. These processes isolate one variant of PSI with a specific ratio of Chl:P700. In this study, we have developed a simple and potentially scalable method for isolating multiple PSI variants using Hydroxyapatite chromatography, which has been well documented in other Photosystem I isolation protocols. By varying the wash conditions, we show that it is possible to change the Chl:P700 ratios. These different PSI complexes were cast into a PSI-Nafion-osmium polymer film that enabled their photoactivity to be measured. Photocurrent increases nearly 400% between highly washed and untreated solutions based on equal chlorophyll content. Importantly, the mild washing conditions remove peripheral Chl and some LHCI without inhibiting the photochemical activity of PSI as suggested by SDS-PAGE analysis. This result could indicate that more P700 could be loaded per surface area for biohybrid devices. Compared with other PSI isolations, this protocol also allows isolation of multiple PSI variants without loss of photochemical activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clorofila / Spinacia oleracea / Complejos de Proteína Captadores de Luz / Complejo de Proteína del Fotosistema I / Electricidad / Luz Idioma: En Revista: Photosynth Res Asunto de la revista: METABOLISMO Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clorofila / Spinacia oleracea / Complejos de Proteína Captadores de Luz / Complejo de Proteína del Fotosistema I / Electricidad / Luz Idioma: En Revista: Photosynth Res Asunto de la revista: METABOLISMO Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos