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Comparative Metal Oxide Nanoparticle Toxicity Using Embryonic Zebrafish.
Wehmas, Leah C; Anders, Catherine; Chess, Jordan; Punnoose, Alex; Pereira, Cliff B; Greenwood, Juliet A; Tanguay, Robert L.
Afiliación
  • Wehmas LC; Department of Environmental and Molecular Toxicology, Environmental Health Sciences Center, Oregon State University, 1007 Agriculture & Life Sciences Building, Corvallis, OR 97331, USA.
  • Anders C; Department of Physics and the Interdisciplinary graduate program in Biomolecular Sciences, Boise State University, 1910 University Drive, Boise, ID 83725, USA.
  • Chess J; Department of Physics and the Interdisciplinary graduate program in Biomolecular Sciences, Boise State University, 1910 University Drive, Boise, ID 83725, USA.
  • Punnoose A; Department of Physics and the Interdisciplinary graduate program in Biomolecular Sciences, Boise State University, 1910 University Drive, Boise, ID 83725, USA.
  • Pereira CB; Department of Statistics, Oregon State University, Corvallis, OR 97331, USA.
  • Greenwood JA; Department of Biochemistry and Biophysics, Environmental Health Sciences Center, Oregon State University, 2011 Agricultural & Life Sciences Building, Corvallis, OR 97331, USA.
  • Tanguay RL; Department of Environmental and Molecular Toxicology, Environmental Health Sciences Center, Oregon State University, 1007 Agriculture & Life Sciences Building, Corvallis, OR 97331, USA.
Toxicol Rep ; 2: 702-715, 2015.
Article en En | MEDLINE | ID: mdl-26029632
Engineered metal oxide nanoparticles (MO NPs) are finding increasing utility in the medical field as anticancer agents. Before validation of in vivo anticancer efficacy can occur, a better understanding of whole-animal toxicity is required. We compared the toxicity of seven widely used semiconductor MO NPs made from zinc oxide (ZnO), titanium dioxide, cerium dioxide and tin dioxide prepared in pure water and in synthetic seawater using a five-day embryonic zebrafish assay. We hypothesized that the toxicity of these engineered MO NPs would depend on physicochemical properties. Significant agglomeration of MO NPs in aqueous solutions is common making it challenging to associate NP characteristics such as size and charge with toxicity. However, data from our agglomerated MO NPs suggests that the elemental composition and dissolution potential are major drivers of toxicity. Only ZnO caused significant adverse effects of all MO particles tested, and only when prepared in pure water (point estimate median lethal concentration = 3.5-9.1 mg/L). This toxicity was life stage dependent. The 24 h toxicity increased greatly (~22.7 fold) when zebrafish exposures started at the larval life stage compared to the 24 hour toxicity following embryonic exposure. Investigation into whether dissolution could account for ZnO toxicity revealed high levels of zinc ion (40-89% of total sample) were generated. Exposure to zinc ion equivalents revealed dissolved Zn2+ may be a major contributor to ZnO toxicity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Toxicol Rep Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Irlanda

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Toxicol Rep Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Irlanda