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The novel secretory protein CGREF1 inhibits the activation of AP-1 transcriptional activity and cell proliferation.
Deng, Weiwei; Wang, Lan; Xiong, Ying; Li, Jing; Wang, Ying; Shi, Taiping; Ma, Dalong.
Afiliación
  • Deng W; Chinese National Human Genome Center, Beijing 100176, China; Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, China; Peking University Center for Human Disease Genomics, Beijing 100191, China.
  • Wang L; Department of Biopharmaceuticals, National Institutes of Food and Drug Control, Beijing 100050, China.
  • Xiong Y; Peking University Center for Human Disease Genomics, Beijing 100191, China.
  • Li J; Peking University Center for Human Disease Genomics, Beijing 100191, China.
  • Wang Y; Chinese National Human Genome Center, Beijing 100176, China; Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, China.
  • Shi T; Chinese National Human Genome Center, Beijing 100176, China; Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, China; Peking University Center for Human Disease Genomics, Beijing 100191, China. Electronic address: tpshi@126.co
  • Ma D; Chinese National Human Genome Center, Beijing 100176, China; Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, China; Peking University Center for Human Disease Genomics, Beijing 100191, China.
Int J Biochem Cell Biol ; 65: 32-9, 2015 Aug.
Article en En | MEDLINE | ID: mdl-26022276
The transcription factor AP-1 plays an important role in inflammation and cell survival. Using a dual-luciferase reporter assay system and a library of 940 candidate human secretory protein cDNA clones, we identified that CGREF1 can inhibit the transcriptional activity of AP-1. We demonstrated that CGREF1 is secreted via the classical secretory pathway through the ER-to-Golgi apparatus. Functional investigations revealed that overexpression of CGREF1 can significantly inhibit the phosphorylation of ERK and p38 MAPK, and suppress the proliferation of HEK293T and HCT116 cells. Conversely, specific siRNAs against CGREF1 can increase the transcriptional activity of AP-1. These results clearly indicated that CGREF1 is a novel secretory protein, and plays an important role in regulation of AP-1 transcriptional activity and cell proliferation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Unión al Calcio / Factor de Transcripción AP-1 Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Biochem Cell Biol Asunto de la revista: BIOQUIMICA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Unión al Calcio / Factor de Transcripción AP-1 Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Biochem Cell Biol Asunto de la revista: BIOQUIMICA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos