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The regeneration and augmentation of bone with injectable osteogenic cell sheet in a rat critical fracture healing model.
Shimizu, Takamasa; Akahane, Manabu; Morita, Yusuke; Omokawa, Shohei; Nakano, Kenichi; Kira, Tsutomu; Onishi, Tadanobu; Inagaki, Yusuke; Okuda, Akinori; Kawate, Kenji; Tanaka, Yasuhito.
Afiliación
  • Shimizu T; Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan. Electronic address: tk-shimi@naramed-u.ac.jp.
  • Akahane M; Department of Public Health, Health Management and Policy, Nara Medical University School of Medicine, Kashihara, Nara 634-8521, Japan.
  • Morita Y; Department of Biomedical Engineering, Doshisha University, Kyotanabe, Kyoto 610-0394, Japan.
  • Omokawa S; Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.
  • Nakano K; Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.
  • Kira T; Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.
  • Onishi T; Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.
  • Inagaki Y; Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.
  • Okuda A; Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.
  • Kawate K; Department of Artificial Joint and Regenerative Medicine, Nara Medical University, Kashihara, Nara 634-8522, Japan.
  • Tanaka Y; Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.
Injury ; 46(8): 1457-64, 2015 Aug.
Article en En | MEDLINE | ID: mdl-26021664
Limitations in the current treatment strategies make cases with compromised bone healing challenging clinical problems. Osteogenic cell sheets (OCSs), fabricated from rat bone marrow stromal cells (BMSCs), contain enriched osteoblasts and extracellular matrix. Here, we evaluated whether the minimally invasive percutaneous injection of OCSs without a scaffold could be used as a treatment to increase bone regeneration in a critical fracture healing model. Critical fracture healing model was created in the femora of 60 male Fischer 344 inbred rats using marrow ablation and periosteal removal. The rats were then randomly divided into two groups. Six hours after fracture, one group received an injection of OCSs (OCS group), while the second group was injected with phosphate-buffered saline (PBS) (control group). Fracture healing was evaluated using radiological, histological, micro-computed tomography (CT) and biomechanical analyses. The radiological and histological evaluations demonstrated enhanced bone regeneration in the OCS group compared with that in the control group. By 12 weeks, the hard callus had been remodelled via recorticalization in the OCS group. By contrast, no fracture union was found in the rats in the control group. Biomechanical testing revealed a significantly higher maximum bending load in the OCS group compared with that in the control group. The results of the present study demonstrate that the injection of entire OCSs can enhance bone regeneration and lead to bony union in a critical fracture healing model. Therefore, this procedure offers a minimally invasive technique to promote hard tissue reconstruction and, in particular, bone repair strategies for cases with compromised bone healing.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración Ósea / Curación de Fractura / Fracturas del Fémur / Fémur / Células Madre Mesenquimatosas / Fracturas no Consolidadas Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Injury Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración Ósea / Curación de Fractura / Fracturas del Fémur / Fémur / Células Madre Mesenquimatosas / Fracturas no Consolidadas Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Injury Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos