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Studies of genotoxicity and mutagenicity of nitroimidazoles: demystifying this critical relationship with the nitro group.
Boechat, Núbia; Carvalho, Alcione S; Salomão, Kelly; Castro, Solange L de; Araujo-Lima, Carlos F; Mello, Francisco V C; Felzenszwalb, Israel; Aiub, Claudia A F; Conde, Taline Ramos; Zamith, Helena P S; Skupin, Rolf; Haufe, Günter.
Afiliación
  • Boechat N; Departamento de Síntese de Fármacos, Farmanguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
  • Carvalho AS; Departamento de Síntese de Fármacos, Farmanguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
  • Salomão K; Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
  • Castro SL; Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
  • Araujo-Lima CF; Departamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
  • Mello FV; Departamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
  • Felzenszwalb I; Departamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
  • Aiub CA; Departamento de Genética e Biologia Molecular, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
  • Conde TR; Departamento de Farmacologia e Toxicologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
  • Zamith HP; Departamento de Farmacologia e Toxicologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
  • Skupin R; Organisch-Chemisches Institut, Westfälischen Wilhelms-Universität Münster, Münster, Germany.
  • Haufe G; Organisch-Chemisches Institut, Westfälischen Wilhelms-Universität Münster, Münster, Germany.
Mem Inst Oswaldo Cruz ; 110(4): 492-9, 2015 Jun.
Article en En | MEDLINE | ID: mdl-26018452
Nitroimidazoles exhibit high microbicidal activity, but mutagenic, genotoxic and cytotoxic properties have been attributed to the presence of the nitro group. However, we synthesised nitroimidazoles with activity against the trypomastigotes of Trypanosoma cruzi, but that were not genotoxic. Herein, nitroimidazoles (11-19) bearing different substituent groups were investigated for their potential induction of genotoxicity (comet assay) and mutagenicity (Salmonella/Microsome assay) and the correlations of these effects with their trypanocidal effect and with megazol were investigated. The compounds were designed to analyse the role played by the position of the nitro group in the imidazole nucleus (C-4 or C-5) and the presence of oxidisable groups at N-1 as an anion receptor group and the role of a methyl group at C-2. Nitroimidazoles bearing NO2 at C-4 and CH3 at C-2 were not genotoxic compared to those bearing NO 2 at C-5. However, when there was a CH3 at C-2, the position of the NO2 group had no influence on the genotoxic activity. Fluorinated compounds exhibited higher genotoxicity regardless of the presence of CH3 at C-2 or NO2 at C-4 or C-5. However, in compounds 11 (2-CH3; 4-NO2; N-CH2OHCH2Cl) and 12 (2-CH3; 4-NO2; N-CH2OHCH2F), the fluorine atom had no influence on genotoxicity. This study contributes to the future search for new and safer prototypes and provide.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella / Trypanosoma cruzi / Daño del ADN / Nitroimidazoles Límite: Animals Idioma: En Revista: Mem Inst Oswaldo Cruz Año: 2015 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella / Trypanosoma cruzi / Daño del ADN / Nitroimidazoles Límite: Animals Idioma: En Revista: Mem Inst Oswaldo Cruz Año: 2015 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil