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A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum.
Gao, Runchi; Zhao, Siwei; Jiang, Xupin; Sun, Yaohui; Zhao, Sanjun; Gao, Jing; Borleis, Jane; Willard, Stacey; Tang, Ming; Cai, Huaqing; Kamimura, Yoichiro; Huang, Yuesheng; Jiang, Jianxin; Huang, Zunxi; Mogilner, Alex; Pan, Tingrui; Devreotes, Peter N; Zhao, Min.
Afiliación
  • Gao R; School of Life Sciences, Yunnan Normal University, Kunming, Yunnan 650500, China. Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, CA 95817, USA. Department of Cell Biology and Anatomy, Johns Hopkins Univers
  • Zhao S; Department of Biomedical Engineering, University of California at Davis, Davis, CA 95616, USA.
  • Jiang X; State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China.
  • Sun Y; Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, CA 95817, USA.
  • Zhao S; School of Life Sciences, Yunnan Normal University, Kunming, Yunnan 650500, China. Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, CA 95817, USA.
  • Gao J; School of Life Sciences, Yunnan Normal University, Kunming, Yunnan 650500, China. Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, CA 95817, USA.
  • Borleis J; Department of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
  • Willard S; Department of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
  • Tang M; Department of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
  • Cai H; Department of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
  • Kamimura Y; Department of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
  • Huang Y; State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China.
  • Jiang J; State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China.
  • Huang Z; School of Life Sciences, Yunnan Normal University, Kunming, Yunnan 650500, China.
  • Mogilner A; Courant Institute and Department of Biology, New York University, 251 Mercer Street, New York, NY 10012, USA.
  • Pan T; Department of Biomedical Engineering, University of California at Davis, Davis, CA 95616, USA.
  • Devreotes PN; Department of Cell Biology and Anatomy, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
  • Zhao M; Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, CA 95817, USA. minzhao@ucdavis.edu.
Sci Signal ; 8(378): ra50, 2015 May 26.
Article en En | MEDLINE | ID: mdl-26012633
Directional cell migration in an electric field, a phenomenon called galvanotaxis or electrotaxis, occurs in many types of cells, and may play an important role in wound healing and development. Small extracellular electric fields can guide the migration of amoeboid cells, and we established a large-scale screening approach to search for mutants with electrotaxis phenotypes from a collection of 563 Dictyostelium discoideum strains with morphological defects. We identified 28 strains that were defective in electrotaxis and 10 strains with a slightly higher directional response. Using plasmid rescue followed by gene disruption, we identified some of the mutated genes, including some previously implicated in chemotaxis. Among these, we studied PiaA, which encodes a critical component of TORC2, a kinase protein complex that transduces changes in motility by activating the kinase PKB (also known as Akt). Furthermore, we found that electrotaxis was decreased in mutants lacking gefA, rasC, rip3, lst8, or pkbR1, genes that encode other components of the TORC2-PKB pathway. Thus, we have developed a high-throughput screening technique that will be a useful tool to elucidate the molecular mechanisms of electrotaxis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Proteínas Protozoarias / Complejos Multiproteicos / Dictyostelium / Proteínas Proto-Oncogénicas c-akt / Serina-Treonina Quinasas TOR Idioma: En Revista: Sci Signal Asunto de la revista: CIENCIA / FISIOLOGIA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Proteínas Protozoarias / Complejos Multiproteicos / Dictyostelium / Proteínas Proto-Oncogénicas c-akt / Serina-Treonina Quinasas TOR Idioma: En Revista: Sci Signal Asunto de la revista: CIENCIA / FISIOLOGIA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos