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Expression analysis of microRNA-125 in patients with polycythemia vera and essential thrombocythemia and correlation with JAK2 allele burden and laboratory findings.
Ferdowsi, S; Atarodi, K; Amirizadeh, N; Toogeh, G; Azarkeivan, A; Shirkoohi, R; Faranoush, M; Vaezi, M; Alimoghaddam, K; Ghavamzadeh, A; Naghadeh, H Teimori; Ghaffari, S H.
Afiliación
  • Ferdowsi S; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
  • Atarodi K; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
  • Amirizadeh N; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
  • Toogeh G; Hematology-Oncology and BMT Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.
  • Azarkeivan A; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
  • Shirkoohi R; Molecular Genetics, Cancer Research Center, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
  • Faranoush M; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
  • Vaezi M; Hematology-Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
  • Alimoghaddam K; Hematology-Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
  • Ghavamzadeh A; Hematology-Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
  • Naghadeh HT; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
  • Ghaffari SH; Hematology-Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Int J Lab Hematol ; 37(5): 661-7, 2015 Oct.
Article en En | MEDLINE | ID: mdl-26011312
INTRODUCTION: The JAK2V617F mutation has emerged in recent years as a diagnostic as well as a treatment target in patients with polycythemia vera (PV) and essential thrombocythemia (ET). The disease phenotype is also influenced by other factors such as microRNA (miRNA) deregulation. The aim of this study was to investigate miR-125 expression level in these patients with those obtained from healthy control subjects and its correlation with JAK2 allele burden and laboratory findings. METHODS: In total, forty patients with a clinical diagnosis of PV and ET were examined at the time of diagnosis. Ten healthy subjects were checked as controls. We performed JAK2 V617F allele burdens measurement and expression analysis of miR-125b-5p, miR-125b-3p, miR-125a-5p, and miR-125a-3p in leukocytes isolated from peripheral blood by quantitative real-time polymerase chain reaction. RESULTS: MiR-125b-5p and miR-125a-5p were upregulated in both patients with PV (P = 0.00 and P = 0.003, respectively) and ET (P = 0.02 and P = 0.002, respectively). In PV group, a significant correlation was observed between miR-125a-5p and platelet counts (P = 0.01, r = 0.531). The correlation between miRNA and JAk2 allele burden was not significant. CONCLUSION: In conclusion, our data indicate that other factors such as aberrant miR-125 expression may influence on the disease phenotype in patients with PV and ET.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Policitemia Vera / Regulación de la Expresión Génica / MicroARNs / Alelos / Janus Quinasa 2 / Frecuencia de los Genes / Trombocitemia Esencial Tipo de estudio: Diagnostic_studies / Observational_studies Límite: Female / Humans / Male Idioma: En Revista: Int J Lab Hematol Asunto de la revista: HEMATOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Policitemia Vera / Regulación de la Expresión Génica / MicroARNs / Alelos / Janus Quinasa 2 / Frecuencia de los Genes / Trombocitemia Esencial Tipo de estudio: Diagnostic_studies / Observational_studies Límite: Female / Humans / Male Idioma: En Revista: Int J Lab Hematol Asunto de la revista: HEMATOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido