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Screening of aptamers specific to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX.
Hung, Lien-Yu; Wang, Chih-Hung; Che, Yu-Jui; Fu, Chien-Yu; Chang, Hwan-You; Wang, Kuan; Lee, Gwo-Bin.
Afiliación
  • Hung LY; Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013.
  • Wang CH; Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013.
  • Che YJ; Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013.
  • Fu CY; Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan 30013.
  • Chang HY; Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan 30013.
  • Wang K; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan 11529.
  • Lee GB; 1] Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013 [2] Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan 30013 [3] Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan 30013.
Sci Rep ; 5: 10326, 2015 May 22.
Article en En | MEDLINE | ID: mdl-25999049
Colorectal cancer (CRC) is the most frequently diagnosed cancer around the world, causing about 700,000 deaths every year. It is clear now that a small fraction of CRC, named colorectal cancer stem cells (CSCs) exhibiting self-renewal and extensive proliferative activities, are hard to be eradicated. Unfortunately, highly specific biomarkers for colorectal CSC (CR-CSCs) are lacking that prohibits the development of effective therapeutic strategies. This study designed and manufactured a novel microfluidic system capable of performing a fully automated cell-based, systematic evolution of ligands by exponential enrichment (SELEX) process. Eight CR-CSC/CRC-specific aptamers were successfully selected using the microfluidic chip. Three of the aptamers showed high affinities towards their respective target cells with a dissociation constant of 27.4, 28.5 and 12.3 nM, which are comparable to that of antibodies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Técnicas Analíticas Microfluídicas / Aptámeros de Nucleótidos Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Técnicas Analíticas Microfluídicas / Aptámeros de Nucleótidos Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido