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Structure of a type IV pilus machinery in the open and closed state.
Gold, Vicki A M; Salzer, Ralf; Averhoff, Beate; Kühlbrandt, Werner.
Afiliación
  • Gold VA; Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany.
  • Salzer R; Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany.
  • Averhoff B; Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany.
  • Kühlbrandt W; Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany.
Elife ; 42015 May 21.
Article en En | MEDLINE | ID: mdl-25997099
Proteins of the secretin family form large macromolecular complexes, which assemble in the outer membrane of Gram-negative bacteria. Secretins are major components of type II and III secretion systems and are linked to extrusion of type IV pili (T4P) and to DNA uptake. By electron cryo-tomography of whole Thermus thermophilus cells, we determined the in situ structure of a T4P molecular machine in the open and the closed state. Comparison reveals a major conformational change whereby the N-terminal domains of the central secretin PilQ shift by ~30 Å, and two periplasmic gates open to make way for pilus extrusion. Furthermore, we determine the structure of the assembled pilus.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Secretina / Modelos Moleculares / Thermus thermophilus / Fimbrias Bacterianas / Complejos Multiproteicos Idioma: En Revista: Elife Año: 2015 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Secretina / Modelos Moleculares / Thermus thermophilus / Fimbrias Bacterianas / Complejos Multiproteicos Idioma: En Revista: Elife Año: 2015 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido