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Genomic instability of osteosarcoma cell lines in culture: impact on the prediction of metastasis relevant genes.
Muff, Roman; Rath, Prisni; Ram Kumar, Ram Mohan; Husmann, Knut; Born, Walter; Baudis, Michael; Fuchs, Bruno.
Afiliación
  • Muff R; Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich, Switzerland.
  • Rath P; Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
  • Ram Kumar RM; Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich, Switzerland.
  • Husmann K; Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich, Switzerland.
  • Born W; Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich, Switzerland.
  • Baudis M; Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
  • Fuchs B; Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich, Switzerland.
PLoS One ; 10(5): e0125611, 2015.
Article en En | MEDLINE | ID: mdl-25992885
BACKGROUND: Osteosarcoma is a rare but highly malignant cancer of the bone. As a consequence, the number of established cell lines used for experimental in vitro and in vivo osteosarcoma research is limited and the value of these cell lines relies on their stability during culture. Here we investigated the stability in gene expression by microarray analysis and array genomic hybridization of three low metastatic cell lines and derivatives thereof with increased metastatic potential using cells of different passages. PRINCIPAL FINDINGS: The osteosarcoma cell lines showed altered gene expression during in vitro culture, and it was more pronounced in two metastatic cell lines compared to the respective parental cells. Chromosomal instability contributed in part to the altered gene expression in SAOS and LM5 cells with low and high metastatic potential. To identify metastasis-relevant genes in a background of passage-dependent altered gene expression, genes involved in "Pathways in cancer" that were consistently regulated under all passage comparisons were evaluated. Genes belonging to "Hedgehog signaling pathway" and "Wnt signaling pathway" were significantly up-regulated, and IHH, WNT10B and TCF7 were found up-regulated in all three metastatic compared to the parental cell lines. CONCLUSIONS: Considerable instability during culture in terms of gene expression and chromosomal aberrations was observed in osteosarcoma cell lines. The use of cells from different passages and a search for genes consistently regulated in early and late passages allows the analysis of metastasis-relevant genes despite the observed instability in gene expression in osteosarcoma cell lines during culture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Óseas / Osteosarcoma / Inestabilidad Genómica / Metástasis de la Neoplasia Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2015 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Óseas / Osteosarcoma / Inestabilidad Genómica / Metástasis de la Neoplasia Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2015 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos