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Cytokine profiles in interstitial fluid from chronic atopic dermatitis skin.
Szegedi, K; Lutter, R; Res, P C; Bos, J D; Luiten, R M; Kezic, S; Middelkamp-Hup, M A.
Afiliación
  • Szegedi K; Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
  • Lutter R; Departments of Respiratory Medicine and Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
  • Res PC; Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
  • Bos JD; Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
  • Luiten RM; Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
  • Kezic S; Coronel Institute of Occupational Health, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
  • Middelkamp-Hup MA; Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
J Eur Acad Dermatol Venereol ; 29(11): 2136-44, 2015 Nov.
Article en En | MEDLINE | ID: mdl-25980674
BACKGROUND: The in vivo levels of inflammatory mediators in chronic atopic dermatitis (AD) skin are not well-defined due to the lack of a non-invasive or minimally invasive sampling technique. OBJECTIVES: To investigate the cytokine milieu in interstitial fluid (ISF) collected from chronic lesional AD skin as compared to ISF from non-lesional AD skin and/or healthy donor skin. METHODS: ISF was obtained using a minimally invasive technique of creating micropores in the skin by a laser, and harvesting ISF through aspiration. We determined the levels of 33 cytokines by Luminex and ELISA in ISF and plasma from sixteen AD patients and twelve healthy individuals. In seven AD patients, we analysed the IL-13, IL-31, IL-17, IL-22 and IFN-γ production by T cells isolated from lesional skin. AD patients were genotyped for the filaggrin gene (FLG)-null mutations 2282del4, R501X, R2447X and S3247X. RESULTS: Twenty-five of 33 examined mediators were detected in the ISF. The levels of IL-1α, IL-1ß, IL-18, IL-1RA, IL-5, IL-13, IL-6, IL-8, TNF-α, RANTES(CCL-5), MIG(CXCL-9), IP-10(CXCL-10), TARC(CCL-17), VEGF and G-CSF showed significant differences between either lesional, non-lesional and/or healthy skin. IP-10 levels in ISF from lesional and non-lesional AD skin showed significant correlation with IP-10 blood levels. IP-10 also showed a significant correlation with clinical severity (SCORAD), as did IL-13. Levels of both IP-10 and IL-13 were more pronounced in patients with FLG-null mutations. Furthermore, FLG-null mutation carriers had more severe AD. CONCLUSION: The presented minimally invasive technique is a valuable tool to determine the in vivo cytokine profile of AD skin.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Citocinas / Líquido Extracelular / Dermatitis Atópica Tipo de estudio: Observational_studies Límite: Humans Idioma: En Revista: J Eur Acad Dermatol Venereol Asunto de la revista: DERMATOLOGIA / DOENCAS SEXUALMENTE TRANSMISSIVEIS Año: 2015 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Citocinas / Líquido Extracelular / Dermatitis Atópica Tipo de estudio: Observational_studies Límite: Humans Idioma: En Revista: J Eur Acad Dermatol Venereol Asunto de la revista: DERMATOLOGIA / DOENCAS SEXUALMENTE TRANSMISSIVEIS Año: 2015 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido