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Colitogenic Bacteroides thetaiotaomicron Antigens Access Host Immune Cells in a Sulfatase-Dependent Manner via Outer Membrane Vesicles.
Hickey, Christina A; Kuhn, Kristine A; Donermeyer, David L; Porter, Nathan T; Jin, Chunsheng; Cameron, Elizabeth A; Jung, Haerin; Kaiko, Gerard E; Wegorzewska, Marta; Malvin, Nicole P; Glowacki, Robert W P; Hansson, Gunnar C; Allen, Paul M; Martens, Eric C; Stappenbeck, Thaddeus S.
Afiliación
  • Hickey CA; Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA; Department of Pediatrics, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.
  • Kuhn KA; Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.
  • Donermeyer DL; Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.
  • Porter NT; Department of Microbiology and Immunology, University of Michigan Medical School, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USA.
  • Jin C; Department of Medical Biochemistry, University of Gothenburg, Box 440, 405 30 Gothenburg, Sweden.
  • Cameron EA; Department of Microbiology and Immunology, University of Michigan Medical School, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USA.
  • Jung H; Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.
  • Kaiko GE; Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.
  • Wegorzewska M; Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.
  • Malvin NP; Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.
  • Glowacki RW; Department of Microbiology and Immunology, University of Michigan Medical School, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USA.
  • Hansson GC; Department of Medical Biochemistry, University of Gothenburg, Box 440, 405 30 Gothenburg, Sweden.
  • Allen PM; Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA. Electronic address: allen@pathology.wustl.edu.
  • Martens EC; Department of Microbiology and Immunology, University of Michigan Medical School, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USA. Electronic address: emartens@umich.edu.
  • Stappenbeck TS; Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA. Electronic address: stappenb@pathology.wustl.edu.
Cell Host Microbe ; 17(5): 672-80, 2015 May 13.
Article en En | MEDLINE | ID: mdl-25974305
Microbes interact with the host immune system via several potential mechanisms. One essential step for each mechanism is the method by which intestinal microbes or their antigens access specific host immune cells. Using genetically susceptible mice (dnKO) that develop spontaneous, fulminant colitis, triggered by Bacteroides thetaiotaomicron (B. theta), we investigated the mechanism of intestinal microbial access under conditions that stimulate colonic inflammation. B. theta antigens localized to host immune cells through outer membrane vesicles (OMVs) that harbor bacterial sulfatase activity. We deleted the anaerobic sulfatase maturating enzyme (anSME) from B. theta, which is required for post-translational activation of all B. theta sulfatase enzymes. This bacterial mutant strain did not stimulate colitis in dnKO mice. Lastly, access of B. theta OMVs to host immune cells was sulfatase dependent. These data demonstrate that bacterial OMVs and associated enzymes promote inflammatory immune stimulation in genetically susceptible hosts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sulfatasas / Bacteroides / Colitis / Vesículas Secretoras / Interacciones Huésped-Patógeno / Antígenos Bacterianos Límite: Animals Idioma: En Revista: Cell Host Microbe Asunto de la revista: MICROBIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sulfatasas / Bacteroides / Colitis / Vesículas Secretoras / Interacciones Huésped-Patógeno / Antígenos Bacterianos Límite: Animals Idioma: En Revista: Cell Host Microbe Asunto de la revista: MICROBIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos