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The tRNA Splicing Endonuclease Complex Cleaves the Mitochondria-localized CBP1 mRNA.
Tsuboi, Tatsuhisa; Yamazaki, Reina; Nobuta, Risa; Ikeuchi, Ken; Makino, Shiho; Ohtaki, Ayumi; Suzuki, Yutaka; Yoshihisa, Tohru; Trotta, Christopher; Inada, Toshifumi.
Afiliación
  • Tsuboi T; From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
  • Yamazaki R; From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
  • Nobuta R; From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
  • Ikeuchi K; From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
  • Makino S; From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
  • Ohtaki A; From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
  • Suzuki Y; the Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
  • Yoshihisa T; the Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan, and.
  • Trotta C; the PTC Therapeutics, South Plainfield, New Jersey 07080.
  • Inada T; From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan, tinada@m.tohoku.ac.jp.
J Biol Chem ; 290(26): 16021-30, 2015 Jun 26.
Article en En | MEDLINE | ID: mdl-25971974
The tRNA splicing endonuclease (Sen) complex is located on the mitochondrial outer membrane and splices precursor tRNAs in Saccharomyces cerevisiae. Here, we demonstrate that the Sen complex cleaves the mitochondria-localized mRNA encoding Cbp1 (cytochrome b mRNA processing 1). Endonucleolytic cleavage of this mRNA required two cis-elements: the mitochondrial targeting signal and the stem-loop 652-726-nt region. Mitochondrial localization of the Sen complex was required for cleavage of the CBP1 mRNA, and the Sen complex cleaved this mRNA directly in vitro. We propose that the Sen complex cleaves the CBP1 mRNA, which is co-translationally localized to mitochondria via its mitochondrial targeting signal.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / ARN Mensajero / Proteínas de Unión al ARN / Proteínas de Saccharomyces cerevisiae / Proteínas Mitocondriales / Endorribonucleasas / Mitocondrias Idioma: En Revista: J Biol Chem Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / ARN Mensajero / Proteínas de Unión al ARN / Proteínas de Saccharomyces cerevisiae / Proteínas Mitocondriales / Endorribonucleasas / Mitocondrias Idioma: En Revista: J Biol Chem Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos