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Insights into the Anaerobic Biodegradation Pathway of n-Alkanes in Oil Reservoirs by Detection of Signature Metabolites.
Bian, Xin-Yu; Mbadinga, Serge Maurice; Liu, Yi-Fan; Yang, Shi-Zhong; Liu, Jin-Feng; Ye, Ru-Qiang; Gu, Ji-Dong; Mu, Bo-Zhong.
Afiliación
  • Bian XY; 1] State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, P.R. China [2] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai 200237, P.R. China.
  • Mbadinga SM; 1] State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, P.R. China [2] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai 200237, P.R. China.
  • Liu YF; 1] State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, P.R. China [2] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai 200237, P.R. China.
  • Yang SZ; 1] State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, P.R. China [2] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai 200237, P.R. China.
  • Liu JF; 1] State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, P.R. China [2] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai 200237, P.R. China.
  • Ye RQ; 1] State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, P.R. China [2] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai 200237, P.R. China.
  • Gu JD; School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, P.R. China.
  • Mu BZ; 1] State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, P.R. China [2] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai 200237, P.R. China.
Sci Rep ; 5: 9801, 2015 May 13.
Article en En | MEDLINE | ID: mdl-25966798
Anaerobic degradation of alkanes in hydrocarbon-rich environments has been documented and different degradation strategies proposed, of which the most encountered one is fumarate addition mechanism, generating alkylsuccinates as specific biomarkers. However, little is known about the mechanisms of anaerobic degradation of alkanes in oil reservoirs, due to low concentrations of signature metabolites and lack of mass spectral characteristics to allow identification. In this work, we used a multidisciplinary approach combining metabolite profiling and selective gene assays to establish the biodegradation mechanism of alkanes in oil reservoirs. A total of twelve production fluids from three different oil reservoirs were collected and treated with alkali; organic acids were extracted, derivatized with ethanol to form ethyl esters and determined using GC-MS analysis. Collectively, signature metabolite alkylsuccinates of parent compounds from C1 to C8 together with their (putative) downstream metabolites were detected from these samples. Additionally, metabolites indicative of the anaerobic degradation of mono- and poly-aromatic hydrocarbons (2-benzylsuccinate, naphthoate, 5,6,7,8-tetrahydro-naphthoate) were also observed. The detection of alkylsuccinates and genes encoding for alkylsuccinate synthase shows that anaerobic degradation of alkanes via fumarate addition occurs in oil reservoirs. This work provides strong evidence on the in situ anaerobic biodegradation mechanisms of hydrocarbons by fumarate addition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias Anaerobias / Petróleo / Alcanos Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Sci Rep Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias Anaerobias / Petróleo / Alcanos Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Sci Rep Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido