Your browser doesn't support javascript.
loading
Downmodulation of vaccine-induced immunity and protection against the intracellular bacterium Francisella tularensis by the inhibitory receptor FcγRIIB.
Franz, Brian J; Li, Ying; Bitsaktsis, Constantine; Iglesias, Bibiana V; Pham, Giang; Sunagar, Raju; Kumar, Sudeep; Gosselin, Edmund J.
Afiliación
  • Franz BJ; Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY 12208, USA.
  • Li Y; Regeneron Pharmaceuticals, 81 Columbia Turnpike, Rensselaer, NY 12144, USA.
  • Bitsaktsis C; Department of Biological Sciences, Seton Hall University, South Orange, NJ 07079, USA.
  • Iglesias BV; Regeneron Pharmaceuticals, 777 Old Saw Mill River Road, Tarrytown, NY 10591, USA.
  • Pham G; Pfizer, 610 Main Street, Cambridge, MA 02139, USA.
  • Sunagar R; Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY 12208, USA.
  • Kumar S; Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY 12208, USA.
  • Gosselin EJ; Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY 12208, USA.
J Immunol Res ; 2015: 840842, 2015.
Article en En | MEDLINE | ID: mdl-25961064
Fc gamma receptor IIB (FcγRIIB) is the only Fc gamma receptor (FcγR) which negatively regulates the immune response, when engaged by antigen- (Ag-) antibody (Ab) complexes. Thus, the generation of Ag-specific IgG in response to infection or immunization has the potential to downmodulate immune protection against infection. Therefore, we sought to determine the impact of FcγRIIB on immune protection against Francisella tularensis (Ft), a Category A biothreat agent. We utilized inactivated Ft (iFt) as an immunogen. Naïve and iFt-immunized FcγRIIB knockout (KO) or wildtype (WT) mice were challenged with Ft-live vaccine strain (LVS). While no significant difference in survival between naïve FcγRIIB KO versus WT mice was observed, iFt-immunized FcγRIIB KO mice were significantly better protected than iFt-immunized WT mice. Ft-specific IgA in serum and bronchial alveolar lavage, as well as IFN-γ, IL-10, and TNF-α production by splenocytes harvested from iFt-immunized FcγRIIB KO, were also significantly elevated. In addition, iFt-immunized FcγRIIB KO mice exhibited a reduction in proinflammatory cytokine levels in vivo at 5 days after challenge, which correlates with increased survival following Ft-LVS challenge in published studies. Thus, these studies demonstrate for the first time the ability of FcγRIIB to regulate vaccine-induced IgA production and downmodulate immunity and protection. The immune mechanisms behind the above observations and their potential impact on vaccine development are discussed.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina A / Receptores de IgG / Francisella tularensis / Anticuerpos Antibacterianos Límite: Animals Idioma: En Revista: J Immunol Res Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Egipto
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina A / Receptores de IgG / Francisella tularensis / Anticuerpos Antibacterianos Límite: Animals Idioma: En Revista: J Immunol Res Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Egipto