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Synergistic Costimulatory Effect of Chlamydia pneumoniae with Carbon Nanoparticles on NLRP3 Inflammasome-Mediated Interleukin-1ß Secretion in Macrophages.
Matsuo, Junji; Nakamura, Shinji; Takeda, Seiji; Ishida, Kasumi; Yamazaki, Tomohiro; Yoshida, Mitsutaka; Chiba, Hitoshi; Hui, Shu-Ping; Yamaguchi, Hiroyuki.
Afiliación
  • Matsuo J; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
  • Nakamura S; Division of Biomedical Imaging Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Takeda S; Health Innovation and Technology Center, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
  • Ishida K; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan Japan Society for the Promotion of Science, Tokyo, Japan.
  • Yamazaki T; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan Japan Society for the Promotion of Science, Tokyo, Japan.
  • Yoshida M; Division of Ultrastructural Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Chiba H; Health Innovation and Technology Center, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan Department of Health Science Technology, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
  • Hui SP; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan Health Innovation and Technology Center, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
  • Yamaguchi H; Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan hiroyuki@med.hokudai.ac.jp.
Infect Immun ; 83(7): 2917-25, 2015 Jul.
Article en En | MEDLINE | ID: mdl-25939513
The obligate intracellular bacterium Chlamydia pneumoniae is not only a causative agent of community-acquired pneumonia but is also associated with a more serious chronic disease, asthma, which might be exacerbated by air pollution containing carbon nanoparticles. Although a detailed mechanism of exacerbation remains unknown, the proinflammatory cytokine interleukin-1ß (IL-1ß) is a critical player in the pathogenesis of asthma. C. pneumoniae induces IL-1ß in macrophages via NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome activation and Toll-like receptor 2/4 (TLR2/4) stimulation. Carbon nanoparticles, such as carbon nanotubes (CNTs), can also evoke the NLRP3 inflammasome to trigger IL-1ß secretion from lipopolysaccharide-primed macrophages. This study assessed whether costimulation of C. pneumoniae with CNTs synergistically enhanced IL-1ß secretion from macrophages, and determined the molecular mechanism involved. Enhanced IL-1ß secretion from C. pneumoniae-infected macrophages by CNTs was dose and time dependent. Transmission electron microscopy revealed that C. pneumoniae and CNTs were engulfed concurrently by macrophages. Inhibitors of actin polymerization or caspase-1, a component of the inflammasome, significantly blocked IL-1ß secretion. Gene silencing using small interfering RNA (siRNA) targeting the NLRP3 gene also abolished IL-1ß secretion. Other inhibitors (K(+) efflux inhibitor, cathepsin B inhibitor, and reactive oxygen species-generating inhibitor) also blocked IL-1ß secretion. Taken together, these findings demonstrated that CNTs synergistically enhanced IL-1ß secretion from C. pneumoniae-infected macrophages via the NLRP3 inflammasome and caspase-1 activation, providing novel insight into our understanding of how C. pneumoniae infection can exacerbate asthma.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carbono / Proteínas Portadoras / Chlamydophila pneumoniae / Interleucina-1beta / Nanopartículas / Inflamasomas / Macrófagos Límite: Humans Idioma: En Revista: Infect Immun Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carbono / Proteínas Portadoras / Chlamydophila pneumoniae / Interleucina-1beta / Nanopartículas / Inflamasomas / Macrófagos Límite: Humans Idioma: En Revista: Infect Immun Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos