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Functional human liver preservation and recovery by means of subnormothermic machine perfusion.
Bruinsma, Bote G; Avruch, James H; Weeder, Pepijn D; Sridharan, Gautham V; Uygun, Basak E; Karimian, Negin G; Porte, Robert J; Markmann, James F; Yeh, Heidi; Uygun, Korkut.
Afiliación
  • Bruinsma BG; Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School.
  • Avruch JH; Transplant Center, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School.
  • Weeder PD; Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School.
  • Sridharan GV; Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School.
  • Uygun BE; Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School.
  • Karimian NG; Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School.
  • Porte RJ; Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University Medical Center Groningen, University of Groningen.
  • Markmann JF; Transplant Center, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School.
  • Yeh H; Transplant Center, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School; hyeh@partners.org.
  • Uygun K; Center for Engineering in Medicine, Dept. of Surgery, Massachusetts General Hospital, Harvard Medical School; korkut.uygun@gmail.com.
J Vis Exp ; (98)2015 Apr 27.
Article en En | MEDLINE | ID: mdl-25938299
There is currently a severe shortage of liver grafts available for transplantation. Novel organ preservation techniques are needed to expand the pool of donor livers. Machine perfusion of donor liver grafts is an alternative to traditional cold storage of livers and holds much promise as a modality to expand the donor organ pool. We have recently described the potential benefit of subnormothermic machine perfusion of human livers. Machine perfused livers showed improving function and restoration of tissue ATP levels. Additionally, machine perfusion of liver grafts at subnormothermic temperatures allows for objective assessment of the functionality and suitability of a liver for transplantation. In these ways a great many livers that were previously discarded due to their suboptimal quality can be rescued via the restorative effects of machine perfusion and utilized for transplantation. Here we describe this technique of subnormothermic machine perfusion in detail. Human liver grafts allocated for research are perfused via the hepatic artery and portal vein with an acellular oxygenated perfusate at 21 °C.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Órganos / Perfusión / Trasplante de Hígado / Hígado Límite: Humans Idioma: En Revista: J Vis Exp Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Órganos / Perfusión / Trasplante de Hígado / Hígado Límite: Humans Idioma: En Revista: J Vis Exp Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos