Enzymatic synthesis of isoamyl butyrate catalyzed by immobilized lipase on poly-methacrylate particles: optimization, reusability and mass transfer studies.
Bioprocess Biosyst Eng
; 38(8): 1601-13, 2015 Aug.
Article
en En
| MEDLINE
| ID: mdl-25935327
Isoamyl butyrate (banana flavor) was synthesized by esterification reaction of isoamyl alcohol and butyric acid in heptane medium. Immobilized Thermomyces lanuginosus lipase (TLL) prepared via physical adsorption on mesoporous poly-methacrylate particles (PMA) was used as biocatalyst. The factors that affect the esterification reaction were optimized by response surface methodology (RSM). Under optimal experimental conditions, maximum ester conversion percentage of 96.1 and 73.6% was reached after 50 and 90 min, respectively, for esterification reaction performed at equimolar ratio alcohol:acid at 500 and 2000 mM of each substrate. Under these experimental conditions, the esterification reaction was not controlled by external and intra-particle mass transfer effects. The product (isoamyl butyrate) was confirmed by proton nuclear magnetic resonance ((1)H NMR) spectroscopy. Reusability tests showed that the biocatalyst retained around 96 and 31% of its initial activity after eight successive esterification cycles performed at 500 and 2000 mM, respectively. The application of the biocatalyst prepared showed to be a promising strategy to catalyze flavor ester synthesis in a non-aqueous medium.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácidos Polimetacrílicos
/
Ascomicetos
/
Butiratos
/
Proteínas Fúngicas
/
Pentanoles
/
Lipasa
Idioma:
En
Revista:
Bioprocess Biosyst Eng
Asunto de la revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Alemania