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An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles.
Medina-Puche, Laura; Molina-Hidalgo, Francisco Javier; Boersma, Maaike; Schuurink, Robert C; López-Vidriero, Irene; Solano, Roberto; Franco-Zorrilla, José-Manuel; Caballero, José Luis; Blanco-Portales, Rosario; Muñoz-Blanco, Juan.
Afiliación
  • Medina-Puche L; Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Science
  • Molina-Hidalgo FJ; Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Science
  • Boersma M; Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Science
  • Schuurink RC; Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Science
  • López-Vidriero I; Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Science
  • Solano R; Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Science
  • Franco-Zorrilla JM; Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Science
  • Caballero JL; Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Science
  • Blanco-Portales R; Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Science
  • Muñoz-Blanco J; Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario 3, Universidad de Córdoba, 14071 Cordoba, Spain (L.M.-P., F.J.M.-H., J.L.C., R.B.-P., J.M.-B.);Department of Plant Physiology, Swammerdam Institute for Life Science
Plant Physiol ; 168(2): 598-614, 2015 Jun.
Article en En | MEDLINE | ID: mdl-25931522
Eugenol is a volatile phenylpropanoid that contributes to flower and ripe fruit scent. In ripe strawberry (Fragaria × ananassa) fruit receptacles, eugenol is biosynthesized by eugenol synthase (FaEGS2). However, the transcriptional regulation of this process is still unknown. We have identified and functionally characterized an R2R3 MYB transcription factor (emission of benzenoid II [FaEOBII]) that seems to be the orthologous gene of PhEOBII from Petunia hybrida, which contributes to the regulation of eugenol biosynthesis in petals. The expression of FaEOBII was ripening related and fruit receptacle specific, although high expression values were also found in petals. This expression pattern of FaEOBII correlated with eugenol content in both fruit receptacle and petals. The expression of FaEOBII was repressed by auxins and activated by abscisic acid, in parallel to the ripening process. In ripe strawberry receptacles, where the expression of FaEOBII was silenced, the expression of cinnamyl alcohol dehydrogenase1 and FaEGS2, two structural genes involved in eugenol production, was down-regulated. A subsequent decrease in eugenol content in ripe receptacles was also observed, confirming the involvement of FaEOBII in eugenol metabolism. Additionally, the expression of FaEOBII was under the control of FaMYB10, another R2R3 MYB transcription factor that regulates the early and late biosynthetic genes from the flavonoid/phenylpropanoid pathway. In parallel, the amount of eugenol in FaMYB10-silenced receptacles was also diminished. Taken together, these data indicate that FaEOBII plays a regulating role in the volatile phenylpropanoid pathway gene expression that gives rise to eugenol production in ripe strawberry receptacles.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Eugenol / Fragaria / Frutas Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Eugenol / Fragaria / Frutas Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos