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Development of a novel matrix-assisted laser desorption/ionization time-of-flight mass spectrum (MALDI-TOF-MS)-based typing method to identify meticillin-resistant Staphylococcus aureus clones.
Ueda, O; Tanaka, S; Nagasawa, Z; Hanaki, H; Shobuike, T; Miyamoto, H.
Afiliación
  • Ueda O; Microbiology Group, MM Business Unit, Siemens Healthcare Diagnostics KK, Tokyo, Japan; Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, Japan.
  • Tanaka S; Department of Clinical Laboratory, Saga University Hospital, Saga, Japan.
  • Nagasawa Z; Department of Medical Technology and Sciences, School of Health Sciences at Fukuoka, International University of Health and Welfare, Fukuoka, Japan.
  • Hanaki H; Kitasato University Research Centre for Anti-infectious Drugs, Tokyo, Japan.
  • Shobuike T; Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, Japan.
  • Miyamoto H; Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, Japan. Electronic address: miyamoth@cc.saga-u.ac.jp.
J Hosp Infect ; 90(2): 147-55, 2015 Jun.
Article en En | MEDLINE | ID: mdl-25922338
BACKGROUND: Mass spectrum analysis enables species- and subspecies-level identification, and can be used as an epidemiological tool in outbreak management. However, its reliability at clonal level has yet to be established. AIM: To establish a matrix-assisted laser desorption/ionization time-of-flight mass-spectrum-based method that enables bacterial clone identification with accuracy equivalent to pulsed-field gel electrophoresis/phage open-reading frame typing (PFGE/POT). METHODS: Meticillin-resistant Staphylococcus aureus (MRSA) was used in this study. Mass spectra were obtained from a standard strain of S. aureus (ATCC29213) and 57 clinically isolated strains, categorized according to POT. Peaks associated with MRSA clone identification (N = 67) were extracted. Based on this peak information, the feasibility of MRSA clone identification was examined by cluster analysis. FINDINGS: In addition to the 58 strains used for peak extraction, mass spectrum analysis of 24 clinically isolated outbreak strains revealed that peak data could be used for successful identification of clones. These typing results were fully consistent with the PFGE and POT results. CONCLUSION: This novel method enables simple and rapid typing with accuracy equivalent to PFGE/POT. This method would be suited to rapid outbreak analysis, offering accurate information to combat infectious diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Bacteriano / Técnicas de Tipificación Bacteriana / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Proteínas Mutantes / Staphylococcus aureus Resistente a Meticilina Tipo de estudio: Prognostic_studies Idioma: En Revista: J Hosp Infect Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Bacteriano / Técnicas de Tipificación Bacteriana / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Proteínas Mutantes / Staphylococcus aureus Resistente a Meticilina Tipo de estudio: Prognostic_studies Idioma: En Revista: J Hosp Infect Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido