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An amplified electrochemical strategy using DNA-QDs dendrimer superstructure for the detection of thymine DNA glycosylase activity.
Liu, Hongying; Lou, Youbing; Zhou, Fei; Zhu, Hao; Abdel-Halim, E S; Zhu, Jun-Jie.
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  • Liu H; State Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China; College of Life Information Science & Instrument Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
  • Lou Y; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China.
  • Zhou F; State Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China.
  • Zhu H; State Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China.
  • Abdel-Halim ES; Petrochemical Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
  • Zhu JJ; State Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China. Electronic address: jjzhu@nju.edu.cn.
Biosens Bioelectron ; 71: 249-255, 2015 Sep 15.
Article en En | MEDLINE | ID: mdl-25913445
A triple-signal amplification strategy was proposed for highly sensitive and selective detection of thymine DNA glycosylase (TDG) by coupling a dendrimer-like DNA label with the electrochemical method and quantum dots (QDs) tagging. The DNA-QDs dendrimer-like superstructure was designed by DNA hybridization and covalent assembling. Benefiting from outstanding performance of the amplification strategy, this assay showed high sensitivity, extraordinary stability, and easy operation. The limit of detection could reach 0.00003 U µL(-1) with a splendid specificity. The TDG content in different concentration of HeLa cell was also determined. This assay opens a new horizon for both qualitative and quantitative detection of TDG, holding great promise for potential application in cancer cell research and clinical diagnostics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Técnicas Biosensibles / Timina ADN Glicosilasa / Puntos Cuánticos / Dendrímeros / Pruebas de Enzimas Tipo de estudio: Diagnostic_studies / Evaluation_studies / Prognostic_studies / Qualitative_research Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Técnicas Biosensibles / Timina ADN Glicosilasa / Puntos Cuánticos / Dendrímeros / Pruebas de Enzimas Tipo de estudio: Diagnostic_studies / Evaluation_studies / Prognostic_studies / Qualitative_research Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido