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The Psp system of Mycobacterium tuberculosis integrates envelope stress-sensing and envelope-preserving functions.
Datta, Pratik; Ravi, Janani; Guerrini, Valentina; Chauhan, Rinki; Neiditch, Matthew B; Shell, Scarlet S; Fortune, Sarah M; Hancioglu, Baris; Igoshin, Oleg A; Gennaro, Maria Laura.
Afiliación
  • Datta P; Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, 07103, USA.
  • Ravi J; Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, 07103, USA.
  • Guerrini V; Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, 07103, USA.
  • Chauhan R; Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, 07103, USA.
  • Neiditch MB; Department of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, 07103, USA.
  • Shell SS; Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, 01605, USA.
  • Fortune SM; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, 021142, USA.
  • Hancioglu B; Department of Bioengineering, Rice University, Houston, TX, 77005, USA.
  • Igoshin OA; Department of Bioengineering, Rice University, Houston, TX, 77005, USA.
  • Gennaro ML; Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, 07103, USA.
Mol Microbiol ; 97(3): 408-22, 2015 Aug.
Article en En | MEDLINE | ID: mdl-25899163
The bacterial envelope integrates essential stress-sensing and adaptive functions; thus, envelope-preserving functions are important for survival. In Gram-negative bacteria, envelope integrity during stress is maintained by the multi-gene Psp response. Mycobacterium tuberculosis was thought to lack the Psp system since it encodes only pspA and no other psp ortholog. Intriguingly, pspA maps downstream from clgR, which encodes a transcription factor regulated by the MprAB-σ(E) envelope-stress-signaling system. clgR inactivation lowered ATP concentration during stress and protonophore treatment-induced clgR-pspA expression, suggesting that these genes express Psp-like functions. We identified a four-gene set - clgR, pspA (rv2744c), rv2743c, rv2742c - that is regulated by clgR and in turn regulates ClgR activity. Regulatory and protein-protein interactions within the set and a requirement of the four genes for functions associated with envelope integrity and surface-stress tolerance indicate that a Psp-like system has evolved in mycobacteria. Among Actinobacteria, the four-gene module occurred only in tuberculous mycobacteria and was required for intramacrophage growth, suggesting links between its function and mycobacterial virulence. Additionally, the four-gene module was required for MprAB-σ(E) stress-signaling activity. The positive feedback between envelope-stress-sensing and envelope-preserving functions allows sustained responses to multiple, envelope-perturbing signals during chronic infection, making the system uniquely suited to tuberculosis pathogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Regulación Bacteriana de la Expresión Génica / Pared Celular / Mycobacterium tuberculosis Tipo de estudio: Prognostic_studies Idioma: En Revista: Mol Microbiol Asunto de la revista: BIOLOGIA MOLECULAR / MICROBIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Regulación Bacteriana de la Expresión Génica / Pared Celular / Mycobacterium tuberculosis Tipo de estudio: Prognostic_studies Idioma: En Revista: Mol Microbiol Asunto de la revista: BIOLOGIA MOLECULAR / MICROBIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido