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Coupling of an enzymatic biofuel cell to an electrochemical cell for self-powered glucose sensing with optical readout.
Pinyou, Piyanut; Conzuelo, Felipe; Sliozberg, Kirill; Vivekananthan, Jeevanthi; Contin, Andrea; Pöller, Sascha; Plumeré, Nicolas; Schuhmann, Wolfgang.
Afiliación
  • Pinyou P; Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, D-44780 Bochum, Germany.
  • Conzuelo F; Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, D-44780 Bochum, Germany.
  • Sliozberg K; Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, D-44780 Bochum, Germany.
  • Vivekananthan J; Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, D-44780 Bochum, Germany.
  • Contin A; Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, D-44780 Bochum, Germany.
  • Pöller S; Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, D-44780 Bochum, Germany.
  • Plumeré N; Center for Electrochemical Sciences (CES) - Molecular Nanostructures, Ruhr-Universität Bochum, D-44780 Bochum, Germany.
  • Schuhmann W; Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, D-44780 Bochum, Germany. Electronic address: wolfgang.schuhmann@rub.de.
Bioelectrochemistry ; 106(Pt A): 22-7, 2015 Dec.
Article en En | MEDLINE | ID: mdl-25892686
A miniaturized biofuel cell (BFC) is powering an electrolyser invoking a glucose concentration dependent formation of a dye which can be determined spectrophotometrically. This strategy enables instrument free analyte detection using the analyte-dependent BFC current for triggering an optical read-out system. A screen-printed electrode (SPE) was used for the immobilization of the enzymes glucose dehydrogenase (GDH) and bilirubin oxidase (BOD) for the biocatalytic oxidation of glucose and reduction of molecular oxygen, respectively. The miniaturized BFC was switched-on using small sample volumes (ca. 60 µL) leading to an open-circuit voltage of 567 mV and a maximal power density of (6.8±0.6) µW cm(-2). The BFC power was proportional to the glucose concentration in a range from 0.1 to 1.0 mM (R(2)=0.991). In order to verify the potential instrument-free analyte detection the BFC was directly connected to an electrochemical cell comprised of an optically-transparent SPE modified with methylene green (MG). The reduction of the electrochromic reporter compound invoked by the voltage and current flow applied by the BFC let to MG discoloration, thus allowing the detection of glucose.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fuentes de Energía Bioeléctrica / Técnicas Biosensibles / Glucosa 1-Deshidrogenasa / Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH / Fenómenos Ópticos / Glucosa Idioma: En Revista: Bioelectrochemistry Asunto de la revista: BIOQUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fuentes de Energía Bioeléctrica / Técnicas Biosensibles / Glucosa 1-Deshidrogenasa / Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH / Fenómenos Ópticos / Glucosa Idioma: En Revista: Bioelectrochemistry Asunto de la revista: BIOQUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Países Bajos