High REDOX RESPONSIVE TRANSCRIPTION FACTOR1 Levels Result in Accumulation of Reactive Oxygen Species in Arabidopsis thaliana Shoots and Roots.

Matsuo, Mitsuhiro; Johnson, Joy Michal; Hieno, Ayaka; Tokizawa, Mutsutomo; Nomoto, Mika; Tada, Yasuomi; Godfrey, Rinesh; Obokata, Junichi; Sherameti, Irena; Yamamoto, Yoshiharu Y; Böhmer, Frank-D; Oelmüller, Ralf

Matsuo, Mitsuhiro; Johnson, Joy Michal; Hieno, Ayaka; Tokizawa, Mutsutomo; Nomoto, Mika; Tada, Yasuomi; Godfrey, Rinesh; Obokata, Junichi; Sherameti, Irena; Yamamoto, Yoshiharu Y; Böhmer, Frank-D; Oelmüller, Ralf.

Afiliación

Matsuo M; Institute of Plant Physiology, Friedrich-Schiller University Jena, Dornburger Straße 159, 07743 Jena, Germany; Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan.
Johnson JM; Institute of Plant Physiology, Friedrich-Schiller University Jena, Dornburger Straße 159, 07743 Jena, Germany.
Hieno A; Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu City 501-1193, Japan.
Tokizawa M; Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu City 501-1193, Japan.
Nomoto M; Center for Gene Research, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Tada Y; Center for Gene Research, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Godfrey R; Institute of Molecular Cell Biology, Center for Molecular Biomedicine, Jena University Hospital, 00743 Jena, Germany; Molecular Cardiology, Department of Cardiovascular Medicine, University Hospital Münster, 48149 Münster, Germany.
Obokata J; Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan.
Sherameti I; Institute of Plant Physiology, Friedrich-Schiller University Jena, Dornburger Straße 159, 07743 Jena, Germany.
Yamamoto YY; Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu City 501-1193, Japan.
Böhmer FD; Institute of Molecular Cell Biology, Center for Molecular Biomedicine, Jena University Hospital, 00743 Jena, Germany.
Oelmüller R; Institute of Plant Physiology, Friedrich-Schiller University Jena, Dornburger Straße 159, 07743 Jena, Germany. Electronic address: b7oera@hotmail.de.

Mol Plant ; 8(8): 1253-73, 2015 Aug.

Article en En | MEDLINE | ID: mdl-25882345

RESUMEN

Redox Responsive Transcription Factor1 (RRTF1) in Arabidopsis is rapidly and transiently upregulated by H2O2, as well as biotic- and abiotic-induced redox signals. RRTF1 is highly conserved in angiosperms, but its physiological role remains elusive. Here we show that inactivation of RRTF1 restricts and overexpression promotes reactive oxygen species (ROS) accumulation in response to stress. Transgenic lines overexpressing RRTF1 are impaired in root and shoot development, light sensitive, and susceptible to Alternaria brassicae infection. These symptoms are diminished by the beneficial root endophyte Piriformospora indica, which reduces ROS accumulation locally in roots and systemically in shoots, and by antioxidants and ROS inhibitors that scavenge ROS. More than 800 genes were detected in mature leaves and seedlings of transgenic lines overexpressing RRTF1; â¼ 40% of them have stress-, redox-, ROS-regulated-, ROS-scavenging-, defense-, cell death- and senescence-related functions. Bioinformatic analyses and in vitro DNA binding assays demonstrate that RRTF1 binds to GCC-box-like sequences in the promoter of RRTF1-responsive genes. Upregulation of RRTF1 by stress stimuli and H2O2 requires WRKY18/40/60. RRTF1 is co-regulated with the phylogenetically related RAP2.6, which contains a GCC-box-like sequence in its promoter, but transgenic lines overexpressing RAP2.6 do not accumulate higher ROS levels. RRTF1 also stimulates systemic ROS accumulation in distal non-stressed leaves. We conclude that the elevated levels of the highly conserved RRTF1 induce ROS accumulation in response to ROS and ROS-producing abiotic and biotic stress signals.

Asunto(s)

Proteínas de Arabidopsis/metabolismo; Arabidopsis/metabolismo; Raíces de Plantas/metabolismo; Brotes de la Planta/metabolismo; Especies Reactivas de Oxígeno/metabolismo; Factores de Transcripción/metabolismo; Acetilcisteína/farmacología; Alternaria/fisiología; Arabidopsis/microbiología; Proteínas de Arabidopsis/genética; Secuencia de Bases; Muerte Celular/efectos de los fármacos; Ditiocarba/farmacología; Regulación de la Expresión Génica de las Plantas/efectos de los fármacos; Genes de Plantas; Luz; Datos de Secuencia Molecular; Moléculas de Patrón Molecular Asociado a Patógenos/metabolismo; Fenotipo; Enfermedades de las Plantas/genética; Enfermedades de las Plantas/microbiología; Raíces de Plantas/efectos de los fármacos; Raíces de Plantas/genética; Raíces de Plantas/efectos de la radiación; Brotes de la Planta/efectos de los fármacos; Brotes de la Planta/efectos de la radiación; Plantas Modificadas Genéticamente; Regiones Promotoras Genéticas/genética; Plantones/efectos de los fármacos; Plantones/genética; Plantones/microbiología; Plantones/efectos de la radiación; Estrés Fisiológico/efectos de los fármacos; Estrés Fisiológico/genética; Estrés Fisiológico/efectos de la radiación; Factores de Transcripción/genética

Palabras clave

H(2)O(2); RAP2.6; REDOX RESPONSIVE TRANSCRIPTION FACTOR1; RRTF1 promoter binding sites; abiotic and biotic stress; reactive oxygen species

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Especies Reactivas de Oxígeno / Arabidopsis / Brotes de la Planta / Raíces de Plantas / Proteínas de Arabidopsis Idioma: En Revista: Mol Plant Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google