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[The detection of occurrence rate of genes coding capability to form pili binding in auto-strains of Escherichia coli].
Klin Lab Diagn ; 60(1): 52-5, 2015 Jan.
Article en Ru | MEDLINE | ID: mdl-25874306
E. coli is a commensal of intestine of the vertebrata. The exchange of genetic material of different types of bacteria between themselves and with other representatives of family of Enterobacteriaceae in intestinal ecosystem results in development of types of normal colibacillus with genetic characteristics of pathogenicity that can serve as a theoretical substantiation to attribute such strains to pathobionts. The entero-pathogenic colibacillus continues be an important cause of diarrhea in children in developing countries. The gene responsible for formation of pili binding is a necessary condition for virulence of entero-pathogenic colibacillus. The polymerase chain reaction was applied to examine 316 strains of different types of E. coli (normal, with weak enzyme activity and hemolytic activity) isolated from healthy children and children with functional disorders of gastro-intestinal tract for presence of genes coding capability to form pill binding. The presence of this gene in different biochemical types of E. coli permits to establish the fact of formation of reservoir of pathogenicity in indigent microbiota of intestinal biocenosis.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Escherichia coli / Proteínas Fimbrias / Diarrea / Escherichia coli / Infecciones por Escherichia coli Tipo de estudio: Diagnostic_studies Límite: Child / Humans Idioma: Ru Revista: Klin Lab Diagn Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2015 Tipo del documento: Article Pais de publicación: Rusia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Escherichia coli / Proteínas Fimbrias / Diarrea / Escherichia coli / Infecciones por Escherichia coli Tipo de estudio: Diagnostic_studies Límite: Child / Humans Idioma: Ru Revista: Klin Lab Diagn Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2015 Tipo del documento: Article Pais de publicación: Rusia