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Productive Infection of Human Embryonic Stem Cell-Derived NKX2.1+ Respiratory Progenitors with Human Rhinovirus.
Jenny, Robert A; Hirst, Claire; Lim, Sue Mei; Goulburn, Adam L; Micallef, Suzanne J; Labonne, Tanya; Kicic, Anthony; Ling, Kak-Ming; Stick, Stephen M; Ng, Elizabeth S; Trounson, Alan; Giudice, Antonietta; Elefanty, Andrew G; Stanley, Edouard G.
Afiliación
  • Jenny RA; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Hirst C; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Lim SM; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Goulburn AL; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Micallef SJ; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Labonne T; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Kicic A; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Ling KM; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Stick SM; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Ng ES; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Trounson A; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Giudice A; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Elefanty AG; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
  • Stanley EG; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre
Stem Cells Transl Med ; 4(6): 603-14, 2015 Jun.
Article en En | MEDLINE | ID: mdl-25873746
UNLABELLED: Airway epithelial cells generated from pluripotent stem cells (PSCs) represent a resource for research into a variety of human respiratory conditions, including those resulting from infection with common human pathogens. Using an NKX2.1-GFP reporter human embryonic stem cell line, we developed a serum-free protocol for the generation of NKX2.1(+) endoderm that, when transplanted into immunodeficient mice, matured into respiratory cell types identified by expression of CC10, MUC5AC, and surfactant proteins. Gene profiling experiments indicated that day 10 NKX2.1(+) endoderm expressed markers indicative of early foregut but lacked genes associated with later stages of respiratory epithelial cell differentiation. Nevertheless, NKX2.1(+) endoderm supported the infection and replication of the common respiratory pathogen human rhinovirus HRV1b. Moreover, NKX2.1(+) endoderm upregulated expression of IL-6, IL-8, and IL-1B in response to infection, a characteristic of human airway epithelial cells. Our experiments provide proof of principle for the use of PSC-derived respiratory epithelial cells in the study of cell-virus interactions. SIGNIFICANCE: This report provides proof-of-principle experiments demonstrating, for the first time, that human respiratory progenitor cells derived from stem cells in the laboratory can be productively infected with human rhinovirus, the predominant cause of the common cold.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhinovirus / Factores de Transcripción / Proteínas Nucleares / Diferenciación Celular / Infecciones por Picornaviridae / Mucosa Respiratoria / Células Madre Embrionarias / Interacciones Huésped-Patógeno Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Stem Cells Transl Med Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhinovirus / Factores de Transcripción / Proteínas Nucleares / Diferenciación Celular / Infecciones por Picornaviridae / Mucosa Respiratoria / Células Madre Embrionarias / Interacciones Huésped-Patógeno Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Stem Cells Transl Med Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido