Rapid super-resolution line-scanning microscopy through virtually structured detection.
Opt Lett
; 40(8): 1683-6, 2015 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-25872047
Virtually structured detection (VSD) has been demonstrated to break the diffraction limit in scanning laser microscopy (SLM). VSD provides an easy, low-cost, and phase-artifact-free strategy to achieve super-resolution imaging. However, practical application of this method is challenging due to a limited image acquisition speed. We report here the combination of VSD and line-scanning microscopy (LSM) to improve the image acquisition speed. A motorized dove prism was used to achieve automatic control of four-angle (i.e., 0°, 45°, 90°, and 135°) scanning, thus ensuring isotropic resolution improvement. Both an optical resolution target and a living frog eyecup were used to verify resolution enhancement.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microscopía Confocal
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Opt Lett
Año:
2015
Tipo del documento:
Article
Pais de publicación:
Estados Unidos