[GAP-43 and its proteolytic fragment in spinal cord cells of rats with experimental autoimmune encephalomyelitis].
Ross Fiziol Zh Im I M Sechenova
; 101(1): 74-84, 2015 Jan.
Article
en Ru
| MEDLINE
| ID: mdl-25868328
The regenerative capacity of the Central Nervous System (CNS) is a key factor implicated in the pathogenesis of neurodegenerative diseases. In the present study, the regenerative capacity of the CNS is considered using one of the markers of regeneration, Growth Associated Protein-43 (GAP-43) and its proteolytic fragment GAP-43-3 in the Experimental Autoimmune Encephalomyelitis (EAE) animal model of multiple sclerosis. The EAE on Wistar rats was characterized as an adequate model of multiple sclerosis, with typical clinical (pares and paralysis) and morphological (infiltration of spinal cord and deformation of motoneurons) disorders. Normally about 60% of GAP-43 is cleaved by m-calpain and stays in the form of GAP-43-3. During severe form of EAE up to 85% of GAP-43 can be found cleaved. We speculated that the cleavage of GAP-43 can play a crucial role for regenerative capacity of CNS during EAE development. Thus the distribution of GAP-43 and GAP-43-3 in the spinal cord was analyzed. The manifestation of clinical signs of EAE has been found to be in correlation with the levels of GAP-43 proteolysis both in the homogenate of the spinal cord and on the spinal cord slices. The immunoreactive staining enabled the observation of the accumulation of GAP-43-3 predominantly in microglial cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Médula Espinal
/
Microglía
/
Proteína GAP-43
/
Encefalomielitis Autoinmune Experimental
/
Neuronas Motoras
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Ru
Revista:
Ross Fiziol Zh Im I M Sechenova
Asunto de la revista:
FISIOLOGIA
Año:
2015
Tipo del documento:
Article
Pais de publicación:
Rusia