Organotypic slice cultures for studies of postnatal neurogenesis.
J Vis Exp
; (97)2015 Mar 04.
Article
en En
| MEDLINE
| ID: mdl-25867138
Here we describe a technique for studying hippocampal postnatal neurogenesis in the rodent brain using the organotypic slice culture technique. This method maintains the characteristic topographical morphology of the hippocampus while allowing direct application of pharmacological agents to the developing hippocampal dentate gyrus. Additionally, slice cultures can be maintained for up to 4 weeks and thus, allow one to study the maturation process of newborn granule neurons. Slice cultures allow for efficient pharmacological manipulation of hippocampal slices while excluding complex variables such as uncertainties related to the deep anatomic location of the hippocampus as well as the blood brain barrier. For these reasons, we sought to optimize organotypic slice cultures specifically for postnatal neurogenesis research.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Órganos
/
Giro Dentado
/
Neurogénesis
/
Hipocampo
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
J Vis Exp
Año:
2015
Tipo del documento:
Article
Pais de publicación:
Estados Unidos