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Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization.
Li, Jinhong; Webster, Margaret A; Wright, Jonathan; Cocker, Jonathan M; Smith, Matthew C; Badakshi, Farah; Heslop-Harrison, Pat; Gilmartin, Philip M.
Afiliación
  • Li J; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
  • Webster MA; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
  • Wright J; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
  • Cocker JM; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
  • Smith MC; The Genome Analysis Centre, Norwich, Research Park, Norwich, NR4 7UH, UK.
  • Badakshi F; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
  • Heslop-Harrison P; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
  • Gilmartin PM; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
New Phytol ; 208(1): 137-48, 2015 Oct.
Article en En | MEDLINE | ID: mdl-25865367
Heteromorphic flower development in Primula is controlled by the S locus. The S locus genes, which control anther position, pistil length and pollen size in pin and thrum flowers, have not yet been characterized. We have integrated S-linked genes, marker sequences and mutant phenotypes to create a map of the P. vulgaris S locus region that will facilitate the identification of key S locus genes. We have generated, sequenced and annotated BAC sequences spanning the S locus, and identified its chromosomal location. We have employed a combination of classical genetics and three-point crosses with molecular genetic analysis of recombinants to generate the map. We have characterized this region by Illumina sequencing and bioinformatic analysis, together with chromosome in situ hybridization. We present an integrated genetic and physical map across the P. vulgaris S locus flanked by phenotypic and DNA sequence markers. BAC contigs encompass a 1.5-Mb genomic region with 1 Mb of sequence containing 82 S-linked genes anchored to overlapping BACs. The S locus is located close to the centromere of the largest metacentric chromosome pair. These data will facilitate the identification of the genes that orchestrate heterostyly in Primula and enable evolutionary analyses of the S locus.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Genes de Plantas / Cromosomas de las Plantas / Flores / Primula / Sitios Genéticos / Desarrollo de la Planta Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Genes de Plantas / Cromosomas de las Plantas / Flores / Primula / Sitios Genéticos / Desarrollo de la Planta Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2015 Tipo del documento: Article Pais de publicación: Reino Unido