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Assessment of circulating serum DNA integrity in colorectal cancer patients.
Yörüker, Ebru E; Özgür, Emre; Keskin, Metin; Dalay, Nejat; Holdenrieder, Stefan; Gezer, Ugur.
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  • Yörüker EE; Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey.
  • Özgür E; Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey.
  • Keskin M; Department of General Surgery, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
  • Dalay N; Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey.
  • Holdenrieder S; Institute of Clinical Chemistry and Pharmacology, University of Bonn, Bonn, Germany.
  • Gezer U; Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey ugurd@istanbul.edu.tr.
Anticancer Res ; 35(4): 2435-40, 2015 Apr.
Article en En | MEDLINE | ID: mdl-25862911
UNLABELLED: Background /Aim: Studies evaluating the integrity of cell-fee DNA (cfDNA) in colorectal cancer (CRC) have led to inconsistent results. Herein, we analyzed the utility of two different DNA integrity indexes (ACTB(384)/ACTB(106) and ALU(247)/ALU(115)) to assess cfDNA fragmentation in Turkish CRC patients. The correlation of circulating nucleosomes (cNUC) with the fragment sizes was also evaluated. MATERIALS AND METHODS: Seventy two CRC patients and 42 CRC-free control individuals were enrolled in the study. cfDNA was analyzed by quantitative polymerase chain reaction (qPCR). RESULTS: While with ALU(247)/ALU(115)there was a small difference between the groups, an approximately 3-fold (median values=0.12 and 0.34) lower integrity was found in the patients using the ACTB(384)/ACTB(106) ratio (p=0.06). The correlation between cNUC and qPCR threshold cycles was much higher for shorter fragments. CONCLUSION: Lower DNA integrity and the predominance of mononuclesomes in serum reveal high fragmentation of cfDNA in CRC patients.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN de Neoplasias / Neoplasias Colorrectales / Actinas / Elementos Alu Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Anticancer Res Año: 2015 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Grecia
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN de Neoplasias / Neoplasias Colorrectales / Actinas / Elementos Alu Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Anticancer Res Año: 2015 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Grecia