Use of radiolabeled antagonist assays for assessing agonism at D2 and D3 dopamine receptors: comparison with functional GTPÎ³S assays.

Zhen, Juan; Antonio, Tamara; Ali, Solav; Neve, Kim A; Dutta, Aloke K; Reith, Maarten E A

Zhen, Juan; Antonio, Tamara; Ali, Solav; Neve, Kim A; Dutta, Aloke K; Reith, Maarten E A.

Afiliación

Zhen J; Department of Psychiatry, New York University School of Medicine, New York, NY, USA. Electronic address: juan.zhen@nyumc.org.
Antonio T; Department of Psychiatry, New York University School of Medicine, New York, NY, USA.
Ali S; Department of Neuroscience and Physiology, New York University School of Medicine, New York, NY, USA.
Neve KA; Research Service, Department of Veterans Affairs Medical Center, Portland, OR, USA.
Dutta AK; Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI, USA.
Reith ME; Department of Psychiatry, New York University School of Medicine, New York, NY, USA; Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, USA.

J Neurosci Methods ; 248: 7-15, 2015 Jun 15.

Article en En | MEDLINE | ID: mdl-25840364

Asunto(s)

Agonistas de Dopamina/farmacología; Evaluación Preclínica de Medicamentos/métodos; Guanosina 5'-O-(3-Tiotrifosfato)/farmacología; Receptores de Dopamina D2/agonistas; Receptores de Dopamina D3/agonistas; Animales; Células CHO; Cricetulus; Células HEK293; Humanos; Receptores de Dopamina D2/genética; Receptores de Dopamina D3/genética; Espiperona/farmacología; Radioisótopos de Azufre; Transfección; Tritio

Palabras clave

Affinity; Binding assay; D(2) receptors; D(3) receptors; Dopamine receptors; Efficacy; Functional assay; GTP shift; [(3)H]spiperone binding; [(35)S] GTP(Î³)S binding

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Guanosina 5'-O-(3-Tiotrifosfato) / Receptores de Dopamina D2 / Agonistas de Dopamina / Evaluación Preclínica de Medicamentos / Receptores de Dopamina D3 Límite: Animals / Humans Idioma: En Revista: J Neurosci Methods Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos

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