Osteogenesis of rat mesenchymal stem cells and osteoblastic cells on strontium-doped nanohydroxyapatite-coated titanium surfaces.
Int J Oral Maxillofac Implants
; 30(2): 461-71, 2015.
Article
en En
| MEDLINE
| ID: mdl-25830407
PURPOSE: The aim of this study was to compare the promotion of osteogenesis in vitro on three types of titanium surfaces: a strontium-hydroxyapatite (Sr-HA)-coated surface, a nano-HA-coated surface, and an uncoated roughened surface. MATERIALS AND METHODS: Sr-HA and HA were placed on disks with a roughened titanium surface by electrochemical deposition. MC3T3-E1 preosteoblast cells and rat bone mesenchymal stem cells were cultured on the Sr-HA, HA-coated, and uncoated roughened disks, and cell adhesion, proliferation, viability, osteogenic differentiation, and formation of mineralized nodules were measured at various time points. RESULTS: The Sr-HA coating produced by a simple electrochemical deposition treatment evidently enhanced the attachment, spreading, alkaline phosphatase activity, and extracellular matrix calcium mineralization of mouse bone mesenchymal stem cells and MC3T3-E1 cells compared with an untreated roughened titanium surface and a nano-HA-coated surface. CONCLUSION: This study suggests that a Sr-doped nano-HA coating produced through electrochemical deposition improves the osteoconductivity of a microrough titanium surface.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteogénesis
/
Estroncio
/
Titanio
/
Células Madre Mesenquimatosas
/
Hidroxiapatitas
Límite:
Animals
Idioma:
En
Revista:
Int J Oral Maxillofac Implants
Asunto de la revista:
ODONTOLOGIA
Año:
2015
Tipo del documento:
Article
Pais de publicación:
Estados Unidos